Expression of Insulin Receptor-1 in Different Tissues of Nile Tilapia and Its Response to Glucose Injection

LIU Hanliang, WANG Jiting, WAN Wenju, SUN Minmin, MENG Xiao, XU Mengmeng

doi:10.3969/j.issn.1006-267x.2017.02.035

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 2: 652 - 662

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.02.035

Molecular Nutrition

Expression of Insulin Receptor-1 in Different Tissues of Nile Tilapia and Its Response to Glucose Injection

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1. College of Animal Science and Technology, Shandong Agricultural University, Taian 271000, China;
2. Department of Basic Medicine, Taishan Medical College, Taian 271000, China

Received date: 2016-07-01

Online published: 2017-02-17

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Abstract

This experiment was conducted to determine the expression of insulin receptor (IR)-1 in different tissues of Nile tilapia (Oreochromis nilotica) and its response to glucose injection. A partial cDNA of IR-1 was cloned from muscle of Nile tilapia by real-time PCR method. The expression differences of IR-1 in muscle, liver and heart were compared by semi-quantitative PCR detection. A total of 160 Nile tilapia with the body weight about 100 g were randomly allotted to 2 groups with 4 replicates per group and 20 fish per replicate. In experimental group, glucose with the dose of 30 mg per 100 g body weight was injected intraperitoneally into the fish, while the fish in control group were injected with 0.7% sterile saline water as the same dose and method. Samples were collected and measured before injection (0 h) and at 1, 3, 6 and 12 h after injection, respectively. The plasma glucose and insulin contents were analyzed, and the relative expression level of IR-1 mRNA in muscle, heart and liver was measured by quantitative real-time PCR detection. The results show as follows:1)the cloned partial cDNA of IR-1 (GenBank accession No. was JN967750) was 1 979 bp, and encoded 548 amino acids. Sequence analysis showed that IR-1 of Nile tilapia had high conservation compared with other species, and had a rich tyrosine kinase characteristic sequence. 2) The higher expression level of IR-1 in muscle, heart and liver was found, the IR-1 expression level in liver and muscle was consistent, while that in heart was relatively lower. 3) The maximum plasma glucose content of experimental group was observed at 1 h after glucose injection which was significantly higher than that of control group (P<0.05), and then began to decrease and returned to normal level at 3 h after injection; the maximum plasma insulin content of experimental group was observed at 3 h after glucose injection which was significantly higher than that of control group (P<0.05), and then began to decrease and returned to normal level at 12 h after injection. The relative expression level of IR-1 mRNA in muscle and liver at 6 h after glucose injection of experimental group was significantly higher than that of control group (P<0.05), and then to return to normal level at 12 h after injection; the relative expression level of IR-1 mRNA in heart had no significant change after glucose injection of experimental group (P>0.05). The results suggest that injecting glucose can instantaneously increase the plasma glucose content of Nile tilapia, but the increase of plasma insulin content is relatively delayed compare with plasma glucose content, and the relative expression level of IR-1 in muscle and liver show a delayed increase relative to plasma insulin content, then the burden of glucose metabolism in Nile tilapia is increased.

Key words： Nile tilapia (Oreochromis nilotica); glucose; insulin; insulin receptor-1

Cite this article

LIU Hanliang, WANG Jiting, WAN Wenju, SUN Minmin, MENG Xiao, XU Mengmeng . Expression of Insulin Receptor-1 in Different Tissues of Nile Tilapia and Its Response to Glucose Injection[J]. Chinese Journal of Animal Nutrition, 2017 , 29(2) : 652 -662 . DOI: 10.3969/j.issn.1006-267x.2017.02.035

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