This experiment was conducted to explore the immunoregulation effects of polysaccharides from pine needles on normal and LPS stimulated mouse peritoneal macrophages. Different concentrations of polysaccharides from pine needles were used in normal and LPS stimulated mouse peritoneal macrophages. The blank control group was added 100 μL RPMI-1640 medium, the positive control group was added 100 μL LPS which the final concentration was 5 μg/mL, the polysaccharides from pine needles groups were added 100 μL of 25, 50, 100, 200 μg/mL polysaccharides from pine needles, and the LPS+polysaccharides from pine needles groups were added the mixture of the same concentration as polysaccharides from pine needles groups and LPS solution which the final concentration was 5 μg/mL and the final volume of the liquid was 200 μL. The thiazolyl blue tetrazolium bromide (MTT) assay was used to tested the cell viability, and the macrophages phagocytic ability was tested by neutral red phagocytosis experiments, the secretory volume of nitrous oxide (NO) was measured by Griess reaction, the secretory volumes of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in culture supernatants were measured by enzyme-linked immunosorbent method (ELISA). The results showed that:1) compared with the blank control group, the relative proliferation rate of macrophages was significantly increased in all polysaccharides from pine needles groups (P < 0.05 or P < 0.01), the neutral red phagocytic rate of macrophages was significantly increased in 50, 100 and 200 μg/mL polysaccharides from pine needles groups (P < 0.05 or P < 0.01), the neutral red phagocytic rate of macrophages was significantly increased in all LPS+polysaccharides from pine needles groups (P<0.01), the NO secretory volume of macrophages was significantly increased in 200 μg/mL polysaccharides from pine needles group (P<0.01), the TNF-α and IL-1β secretory volumes of macrophages were significantly increased in 50, 100 and 200 μg/mL polysaccharides from pine needles groups (P < 0.05 or P < 0.01), the IL-10 secretory volume of macrophages was significantly decreased in 50, 100 and 200 μg/mL polysaccharides from pine needles groups (P<0.01). 2) Compared with the positive control group, the relative proliferation rate of macrophages was significantly decreased in all polysaccharides from pine needles groups (P<0.01), the neutral red phagocytic rate of macrophages was significantly increased in 100 and 200 LPS+polysaccharides from pine needles groups (P<0.01), the NO secretory volume of macrophages was significantly increased in 50, 100 and 200 μg/mL LPS+polysaccharides from pine needles group (P<0.01), the TNF-α secretory volume of macrophages was significantly increased in all LPS+polysaccharides from pine needles groups (P < 0.05 or P < 0.01), the IL-1β secretory volume of macrophages was significantly increased in 50, 100 and 200 μg/mL LPS+polysaccharides from pine needles groups (P < 0.05 or P < 0.01), the IL-10 secretory volume of macrophages was significantly decreased in 100 and 200 μg/mL LPS+polysaccharides from pine needles groups (P<0.01). It is concluded that polysaccharides from pine needles can play a role in proinflammatory, thereby regulate the immune function of macrophages, thus enhancing the ability of anti-disease.