Molecular Nutrition

Associative Effects of Corn Stalk, Rice Straw, Corn Stalk Silage and Concentrate Evaluated by Gas Production Technique in Vitro

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  • 1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001, China;
    2. College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China

Received date: 2016-07-12

  Online published: 2017-02-17

Abstract

This experiment was conducted to investigate the associative effects of corn stalk (CS), rice straw (RS), corn stalk silage (CSS) and concentrate (CC). Single factor experiment was started with the screening for the optimal proportion of combination of CS and RS (CS-RS), which was subsequently recombined with CSS to screen the optimal proportion of combination of CS-RS and CSS (CS-RS-CSS), finally, CC was added to screen the optimal proportion of combination of CS-RS-CSS and CC. Every combination was tested in vitro with the proportions of 100.0:0, 80.0:20.0, 60.0:40.0, 50.0:50.0, 40.0:60.0, 20.0:80.0 and 0:100.0 with 3 replicates, respectively. In vitro gas production method was performed to analyze 48 h gas production, dry matter disappearance rate (DMD), pH, and the concentrations of microbial crude protein (MCP), ammonia nitrogen (NH3-N) and volatile fat acids (VFAs), as well as to calculate single factor associative effects index (SFAEI) and multiply factors associative effects index (MFAEI). The results showed as follows:1) feeds combined at different proportions had significant effects on gas production (P < 0.05 or P < 0.01), and SFAEI of gas production for CS:RS, CS-RS:CSS and CS-RS-CSS:CC reached the biggest at the proportions of 60.0:40.0, 40.0:60.0 and 20.0:80.0, respectively; 2) feeds combined at different proportions had significant effects on DMD (P < 0.05 or P < 0.01), and the optimal proportions of CS:RS, CS-RS:CSS and CS-RS-CSS:CC were 50.0:50.0, 40.0:60.0 and 20.0:80.0 according to the SFAEI, respectively; 3) different proportions of CS-RS-CSS and CC had significant effects on pH (P<0.01); 4) feeds combined at different proportions had significant effects on MCP concentration (P < 0.05 or P < 0.01), and the optimal proportions of CS:RS, CS-RS:CSS and CS-RS-CSS:CC were 80.0:20.0, 40.0:60.0 and 20.0:80.0 according to the SFAEI, respectively; 5) feeds combined at different proportions had significant effects on NH3-N concentration (P < 0.05 or P < 0.01) changing from 20.20 to 31.59 mg/dL; 6) there were no significant differences in acetic acid/propionic acid among different proportions of combinations of CS and RS, and in butyric concentration among different proportions of combinations of CS-RS-CSS and CC (P>0.05), and feeds combined at different proportions had significant effects on the rest VFA and TVFA concentrations (P < 0.05 or P < 0.01). Using MFAEI to assess the optimal results of associative effect, it is conclude that the optimal proportions are as follows:CS:RS is 60.0:40.0; CS:RS:CSS is 24.0:16.0:60.0; CS:RS:CSS:CC is 9.6:6.4:24.0:60.0.

Cite this article

HAN Xiaomin, CAO Yufeng, LI Qiufeng, GAO Yanxia, LI Yan, LI Jianguo . Associative Effects of Corn Stalk, Rice Straw, Corn Stalk Silage and Concentrate Evaluated by Gas Production Technique in Vitro[J]. Chinese Journal of Animal Nutrition, 2017 , 29(2) : 699 -711 . DOI: 10.3969/j.issn.1006-267x.2017.02.040

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