A Research in Oxidative Stress and Damage of Breeding Goats

YU Sijia, SHI Donghui, ZHU Yong, XU Jianxiong, XU Weina, ZHANG Jing

doi:10.3969/j.issn.1006-267x.2017.03.011

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 3: 814 - 823

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.011

Molecular Nutrition

A Research in Oxidative Stress and Damage of Breeding Goats

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1. Shanghai Key Laboratory of Veterinary Bio-Tech, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China;
2. Shanghai Chongming Animal Disease Prevention and Control Center, Shanghai 202150, China

Received date: 2016-09-02

Online published: 2017-03-09

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Abstract

This study was conducted to investigate the oxidative stress and damage status of breeding goats at different breeding stages and different parities. In test 1, Chongming white goats with similar body weight and physical condition at 2 to 3 parities at different breeding stages were used, and the stages were as follows:non-pregnant stage, pregnant stage (30, 60 and 120 d), the parturition day and lactation stage (21 d), and five from each were used; in test 2, Chongming white goats at different parities were used, and the parities were nulliparity,1 parity, 2 to 3 parities, 4 to 5 parities and 6 and above parities, and five from each were used. Blood sample was collected to analysis serum free radical metabolism indexes[cortisol and hydrogen peroxide (H₂O₂) contents], antioxidant indexes[total antioxidant capacity (T-AOC), and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD)] and oxidative damage indexes[maleic dialdehyde (MDA), 8-iso prostaglandin (8-ISO-PGF2α), 8-hydroxydeoxyguanosine (8-OHDG), and protein carbonyl (PC) contents]. The results showed as follows:in test 1, from non-pregnant stage to lactation stage, serum contents of cortisol and H₂O₂ were firstly increased and then decreased, and reached the highest values on the parturition day, which were significantly higher than those at non-pregnant stage (P<0.05); serum T-AOC and SOD activity were gradually increased, and lactation stage (21 d) was significantly higher than non-pregnant stage (P<0.05); serum contents of MDA, 8-ISO-PGF2α and PC were firstly increased and then tended to be stable, and reached the highest values on the parturition day, which were significantly higher than those at non-pregnant stage (P<0.05); serum 8-OHDG content was gradually increased, and reached the highest value at lactation stage (21 d), which was significantly higher than that at non-pregnant stage (P<0.05). In test 2, with the increase of parities, there was no significant changes of serum cortisol content (P>0.05), and serum H₂O₂ content at 4 to 5 parities and 6 and above parities was significantly higher than that at the other parities (P<0.05); there was no significant change of serum T-AOC (P<0.05), but serum activities of SOD and GSH-Px were gradually decreased, and 6 and above parities were significantly decreased compared with nulliparity (P<0.05); serum contents of MDA, 8-ISO-PGF2α, 8-OHDG and PC were gradually increased, and 6 and above parities were significantly higher than parities (P<0.05). In conclusion, with the development of pregnancy, free radical metabolism of goats is gradually enhanced, meanwhile, antioxidant capacity is also improved to keep the oxidant-antioxidant system balance; but due to the proliferation of reactive oxygen species from late-pregnant stage to lactation stage, the balance is broken, and strong oxidative damage is caused. with the increase of parities, the antioxidant capacity of goats get weak, high parities goats suffer strong oxidative damage.

Key words： breeding goat; pregnancy; parity; oxidative stress

Cite this article

YU Sijia, SHI Donghui, ZHU Yong, XU Jianxiong, XU Weina, ZHANG Jing . A Research in Oxidative Stress and Damage of Breeding Goats[J]. Chinese Journal of Animal Nutrition, 2017 , 29(3) : 814 -823 . DOI: 10.3969/j.issn.1006-267x.2017.03.011

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