Effects of Carbohydrate Type and Level on Growth Performance, Serum Biochemical Indices, Hepatic Glycometabolism-Related Enzyme Activities and Hepatic Glycogen Content of Large Yellow Croaker (Larimichthys crocea Richardson)

MA Hongna, WANG Mengqiang, LU You, YUAN Ye, SUN Peng, ZHOU Qicun

doi:10.3969/j.issn.1006-267x.2017.03.012

2017 , Vol. 29 >Issue 3: 824 - 835

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.012

Molecular Nutrition

Effects of Carbohydrate Type and Level on Growth Performance, Serum Biochemical Indices, Hepatic Glycometabolism-Related Enzyme Activities and Hepatic Glycogen Content of Large Yellow Croaker (Larimichthys crocea Richardson)

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Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2016-09-08

Online published: 2017-03-09

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Abstract

An 8-week feeding trial was conducted to evaluate the effects of carbohydrate type and level on growth performance, whole body and muscle conventional components, serum biochemical indices, hepatic glycometabolism-related enzyme activities and hepatic glycogen content of large yellow croaker (Larimichthys crocea Richardson). Using a 2×3 two-factor experiment design, five isonitrogenous and isolipidic experimental diets were formulated to contain three carbohydrate levels (0, 15% and 30%), and glucose and wheat starch were used as carbohydrate sources, respectively. Each diet was randomly assigned to 3 replicates and each replicate had 50 large yellow croakers with the initial body weight of (8.53±0.07) g. The results showed that the final body weight, weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR) were significantly affected by dietary carbohydrate type and level and their interaction (P<0.05). The final body weight, WGR and SGR significantly decreased with dietary glucose level increasing from 0 to 30% (P<0.05); however, the final weight, WGR and SGR significantly increased with dietary wheat starch level increasing from 0 to 15% and then significantly decreased with dietary wheat starch level increasing from 15% to 30% (P<0.05). FCR significantly increased with of dietary glucose level increasing (P<0.05), but it significantly decreased with dietary wheat starch level increasing from 0 to 15% (P<0.05) and significantly increased with dietary wheat starch level increasing from 15% to 30% (P<0.05). Total protein (TP), total cholesterol (TC), triglyceride (TG) and glucose contents in serum were significantly affected by the interaction of dietary carbohydrate type and level (P<0.05). Glucose content in serum was firstly decreased and then increased with dietary glucose level increasing, and the difference was significant among groups (P<0.05). With dietary wheat starch level increasing, glucose content in serum had a decrease trend, and it in 30% wheat starch group was significantly lower than that in 0 wheat starch group (P<0.05). Hepatic glucokinase (GK), phosphofructokinase (PFK) and glucose-6-phosphatase (G6Pase) activities and hepatic glycogen content were significantly affected by the interaction of dietary carbohydrate type and level (P<0.05). Hepatic glycogen content had a continuously increase with dietary glucose level increasing, but it increased at first and then decreased with dietary wheat starch increasing. Fish fed the diets with wheat starch had significantly higher hepatic glycogen content than those fed the diets with glucose under the 15% or 30% level (P<0.05). These results demonstrate that compared with glucose, diet with wheat starch can make large yellow croaker has the ability to maintain a relatively constant concentration of blood glucose by adjusting hepatic glycometabolism-related enzyme activities and hepatic glycogen content, and the growth performance of large yellow croaker can be improved when the diet with 15% wheat starch.

Key words： large yellow croaker (Larmichthys crocea Richardson); carbohydrate; growth performance; glycometabolism-related enzymes; hepatic glycogen

Cite this article

MA Hongna, WANG Mengqiang, LU You, YUAN Ye, SUN Peng, ZHOU Qicun . Effects of Carbohydrate Type and Level on Growth Performance, Serum Biochemical Indices, Hepatic Glycometabolism-Related Enzyme Activities and Hepatic Glycogen Content of Large Yellow Croaker (Larimichthys crocea Richardson)[J]. Chinese Journal of Animal Nutrition, 2017 , 29(3) : 824 -835 . DOI: 10.3969/j.issn.1006-267x.2017.03.012

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