Effects of Methionine on Expression of Genes and Proteins Related with Milk Fat Synthesis in Bovine Mammary Epithelial Cells

ZHAO Yanli, CHEN Lu, SHI Binlin, GUO Xiaoyu, YAN Sumei

doi:10.3969/j.issn.1006-267x.2017.03.029

2017 , Vol. 29 >Issue 3: 961 - 969

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.029

Molecular Nutrition

Effects of Methionine on Expression of Genes and Proteins Related with Milk Fat Synthesis in Bovine Mammary Epithelial Cells

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Collage of Animal Science, Inner Mongolia Agriculture University, Hohhot 010018, China

Received date: 2016-08-30

Online published: 2017-03-09

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Abstract

This experiment was to study the effects of methionine (Met) on expression of genes and proteins related with milk fat synthesis in bovine mammary epithelial cells (BMECs), in order to investigate the mechenism of Met regulating milk fat synthesis. The third generation of BMECs were divided into six treatments with six replicates per treatment, and cultured in culture mediums with 0.13, 0.26, 0.39, 0.52, 0.65 and 0.78 mmol/L Met, respectively. The triglyceride (TG) content, the relative expression levels of genes related with milk fat synthesis, as well as the protein relative expression levels of peroxisome proliferator-activated receptor-γ (PPARγ) and sterol regulatory element binding protein 1 (SREBP1) in BMECs after 48 h incubation at 37℃ and 5% CO₂ were detected. The results showed that Met concentration had no significant effect on TG content in BMECs (P>0.05). The gene relative expression levels of fatty acid-binding protein 3 (FABP3), acetyl-CoA carboxylase A (ACACA) and PPARγ in BMECs of 0.52 to 0.78 mmol/L Met treatments were significantly higher than those of other treatments (P<0.05). The gene relative expression level of lipoprotein lipase (LPL) in BMECs of 0.26 to 0.39 mmol/L Met treatments was higher, which significantly higher than that of 0.65 to 0.78 mmol/L treatments (P<0.05). The gene relative expression level of fatty acid synthase (FASN) in BMECs of 0.39 to 0.52 mmol/L Met treatments was higher, and it of 0.52 mmol/L Met treatment was significantly higher than that of 0.13 to 0.26 mmol/L and 0.65 to 0.78 mmol/L Met treatments (P<0.05). Met concentration could significantly affect the gene expression of SREBP1 and 1-acylglycerol-3-phosphate O-acyltransferase 6 (AGPAT6) and the protein expression of SREBP1 and PPARγ in BMECs (P<0.05), and the highest relative expression levels of them were all observed in 0.26 mmol/L Met treatment. These results demonstrate that Met concentration affects the expression of genes related with long-chain fatty acids uptake and de novo synthesis of fatty acids, as well as the gene and protein expression of SREBP1 and PPARγ which were milk fat synthesis regulatory factors in BMECs. Met with the concentrations of 0.26 to 0.52 mmol/L can produce the better effects for long-chain fatty acids uptake and de novo synthesis of fatty acids in BMECs.

Key words： dairy cow; bovine mammary epithelial cells; methionine; milk fat

Cite this article

ZHAO Yanli, CHEN Lu, SHI Binlin, GUO Xiaoyu, YAN Sumei . Effects of Methionine on Expression of Genes and Proteins Related with Milk Fat Synthesis in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2017 , 29(3) : 961 -969 . DOI: 10.3969/j.issn.1006-267x.2017.03.029

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