Screening and Identification of Chitinase Producing Strain and Its Inhibition Action to Dominant Moulds in Corn Stalk

ZHANG Liyang, ZHANG Xingyi, ZHANG Wei, LIN Cong, ZHANG Yonggen

doi:10.3969/j.issn.1006-267x.2017.03.030

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 3: 970 - 978

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.030

Molecular Nutrition

Screening and Identification of Chitinase Producing Strain and Its Inhibition Action to Dominant Moulds in Corn Stalk

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College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2016-09-01

Online published: 2017-03-09

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Abstract

The objective of this experiment was to inhibit the growth of dominant moulds in corn stalk using chitinase producing strain, and provide a reference for improving the utilization efficiency of corn stalk. Chitinase producing strain of BS-1 was screened from corn stalk using colloidal chitin medium. At the same time, the dominant moulds were isolated and purified. Identification of strain BS-1 and dominant moulds were carried out through morphological observation and 16S rDNA or 18S rDNA sequence analysis. In addition, the chitinase activity of strain BS-1 was assayed using 3,5-dinitrosalicylic acid colorimetric method after 24, 48, 72, 96, 120, 144 and 168 h of fermentation and the inhibition action of fermentation liquid of strain BS-1 to dominant moulds was detected by oxford cup method. The results showed as follows:strain BS-1 isolated from corn stalk was Bacillus subtilis and four dominant moulds were Mucor circinelloides, Fusarium oxysporum, Aspergillus oryzae and Aspergillus niger, respectively. After 120 h of fermentation at 37℃, the highest chitinase activity of strain BS-1 was obtained to be 3.23 U/mL. The fermentation liquid of strain BS-1 showed obvious inhibition to four dominant moulds from corn stalk, the diameter of inhibition zone was 18.13, 18.48, 17.55 and 15.68 mm, respectively. In conclusion, the screened chitinase producing Bacillus subtilis BS-1 can effectively inhibit the growth of dominant moulds of Mucor circinelloides, Fusarium oxysporum, Aspergillus oryzae and Aspergillus niger from corn straw.

Key words： corn stalk; dominant moulds; antifungal; chitinase; Bacillus subtilis

Cite this article

ZHANG Liyang, ZHANG Xingyi, ZHANG Wei, LIN Cong, ZHANG Yonggen . Screening and Identification of Chitinase Producing Strain and Its Inhibition Action to Dominant Moulds in Corn Stalk[J]. Chinese Journal of Animal Nutrition, 2017 , 29(3) : 970 -978 . DOI: 10.3969/j.issn.1006-267x.2017.03.030

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