Effects of Radix pseudostellariae Stem and Leaf Polysaccharide on Intestinal Immune Function, Intestinal Mucosal Morphology and Cecum Contents Flora of Weaned Piglets

CHEN Lingfeng, CAI Xubin, TAN Xinzhu, LIN Guoxu, MA Yufang, HUANG Yifan

doi:10.3969/j.issn.1006-267x.2017.03.035

2017 , Vol. 29 >Issue 3: 1012 - 1020

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.035

Molecular Nutrition

Effects of Radix pseudostellariae Stem and Leaf Polysaccharide on Intestinal Immune Function, Intestinal Mucosal Morphology and Cecum Contents Flora of Weaned Piglets

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1. Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Putian Youlike Agriculture and Animal Husbandry Developing Co., Ltd., Putian 351111, China

Received date: 2016-08-24

Online published: 2017-03-09

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Abstract

This study was conducted to investigate the effects of dietary Radix pseudostellariae stem and leaf polysaccharide (RpSLp) on intestinal immune function, intestinal mucosal morphology and cecum contents flora of weaned piglets. One hundred and twenty 25-day-old weaned piglets (Large White×Landrace) with similar average body weight were selected and randomly allocated into four groups with three replicates per group and 10 piglets per replicate. Piglets in the control group were fed a basal diet, and the others in the experimental groups were fed the basal diets supplemented with 500 (0.05% polysaccharide group), 1 000 (0.10% polysaccharide group) and 1 500 RpSLp (0.15% polysaccharide group), respectively. The experiment lasted for 30 days. The results showed as follows:compared with the control group, the duodenum interleukin-4 (IL-4) content in experimental groups was significantly increased (P<0.05), the duodenum interferon-γ (IFN-γ) content in 0.10% polysaccharide group was significantly increased (P<0.05), the ileum secretory immunoglobulin A (SIgA) content in 0.10% and 0.15% polysaccharide groups was significantly improved (P<0.05); both duodenum and jejunum villi height in 0.10% and 0.15% polysaccharide groups was significantly increased (P<0.01 or P<0.01); and the villi height to crypt depth (V/C) value was also significantly increased (P<0.05); the ileum villi height in 0.15% polysaccharide group was significantly improved (P<0.05); the number of Escherichia coli in cecum contents in experimental groups was significantly decreased (P<0.05), but the Lactobacillus number was significantly increased (P<0.05). It is concluded that dietary RpSLp can improve intestinal immune function, increase the intestinal villi height and V/C calue, regulate flora structure of cecum contents, and keep the balance for intestinal microecology of weaned piglets. The optimum additive amount of RpSLp is 0.10%.

Key words： Radix pseudostellariae stem and leaf polysaccharide; weaned piglets; intestinal immune function; intestinal mucosal morphology; cecum contents flora

Cite this article

CHEN Lingfeng, CAI Xubin, TAN Xinzhu, LIN Guoxu, MA Yufang, HUANG Yifan . Effects of Radix pseudostellariae Stem and Leaf Polysaccharide on Intestinal Immune Function, Intestinal Mucosal Morphology and Cecum Contents Flora of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2017 , 29(3) : 1012 -1020 . DOI: 10.3969/j.issn.1006-267x.2017.03.035

References

[1] BISCHOFF S C.‘Gut health’:a new objective in medicine[J].BMC Medicine,2011,9:24.

[2] LAMBERT G P.Stress-induced gastrointestinal barrier dysfunction and its inflammatory effects[J].Journal of Animal Science,2009,87(S14):101-108.

[3] 李同洲,薛凌峰,臧素敏,等.香菇多糖对仔猪生产性能、部分血清激素和免疫指标的影响[J].动物营养学报,2009,21(1):101-106.

[4] 韩杰,边连全,刘显军,等.刺五加多糖对断奶仔猪血液免疫指标的影响[J].动物营养学报,2012,24(12):2444-2449.

[5] 杨兵,夏先林,施晓丽,等.熟地黄多糖对断奶仔猪抗氧化性能和免疫性能的影响[J].江苏农业学报,2012,28(4):787-791.

[6] 杨侃侃,边连全,刘显军,等.刺五加多糖对断奶仔猪生长性能、血清免疫指标及粪便微生物菌群的影响[J].动物营养报,2013,25(3):628-634.

[7] 国家药典委员会.中华人民共和国药典:一部[M].2010版.北京:中国医药科技出版社,2010:62-63.

[8] 严胜泽,马玉芳,廖吕燕,等.太子参多糖对环磷酰胺所致肠道黏膜损伤小鼠SIgA、IL-2、IL-6含量的影响[J].中国畜牧兽医,2015,42(5):1187-1192.

[9] 雷松波,池春梅,金其全,等.太子参茎叶对断乳仔猪抗氧化和抗应激能力的影响[J].福建畜牧兽医,2014, 36(6):28-30.

[10] 曾丽娜,袁玉虹,黄淑燕,等.不同采收时间、不同入药部位太子参有效药用成分含量的比较[J].福建林业,2014(2):25-26.

[11] 许丛丛,陈小连,朱丽慧,等.复合抗氧化剂对早期断奶仔猪肠道主要菌群的影响[J].饲料研究,2012(4):4-7.

[12] GEBERT A,GÖKE M,ROTHKÖTTER H J,et al.The mechanisms of antigen uptake in the small and large intestines:the roll of the M cells for the initiation of immune responses[J].Zeitschrift Für Gastroenterologie,2000,38(10):855-872.

[13] 张源淑,邓艳,宋晓丹,等.酪啡肽及其酪蛋白水解肽对早期断奶仔猪分泌型免疫球蛋白A和细胞因子水平的影响[J].动物营养学报,2008,20(2):196-199.

[14] 张大鹏,周联,张志敏,等.四君子汤总多糖对免疫抑制小鼠肠道SIgA的影响及其机制研究[J].中药新药与临床药理,2010,21(1):11-14.

[15] 王庭祥,王庭欣,刘峥颢,等.海带多糖对小鼠肠黏膜组织SIgA的影响[J].安徽农业科学,2009,37(12):5515,5520.

[16] 姬云丽,凌虹,于新慧,等.当归补血汤多糖对隐孢子虫感染小鼠肠道局部免疫功能的影响[J].牡丹江医学院学报,2007,28(6):6-8.

[17] 张圣方,赵龙玉,赵凤春,等.泰山蛹虫草多糖对免疫抑制小鼠肠道菌群及分泌型免疫球蛋白A的影响[J].食品科学,2015,36(5):148-152.

[18] DUNSFORD B R,KNABE D A,HAENSLY W E.Effect of dietary soybean meal on the microscopic anatomy of the small intestine in the early-weaned pig[J].Journal of Animal Science,1989,67(7):1855-1863.

[19] 李鹏.半乳甘露寡糖对兔小肠黏膜结构及黏膜免疫相关指标的影响[D].硕士学位论文.北京:中国农业大学,2007:119-121.

[20] 王留,王向国.大枣多糖对保育猪肠道微生物菌群及肠道组织形态的影响[J].养猪,2014(6):7-8.

[21] 黄玉章,林旋,王全溪,等.黄芪多糖对罗非鱼肠绒毛形态结构及肠道免疫细胞的影响[J].动物营养学报,2010,22(1):108-116.

[22] 石玉祥,闫金坤,王雪敏.枸杞多糖对小鼠肠道上皮内淋巴细胞和杯状细胞数量、分布及对IL-2水平影响[J].食品科学,2011(13):318-320.

[23] 冯澜,李绍民,代立娟,等.马齿苋多糖对溃疡性结肠炎小鼠肠黏膜细胞因子及肠道菌群的影响[J].中国微生态学杂志,2015,27(2):139-142.

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