Molecular Nutrition

Effects of Tea Saponin on Immune Function of Dairy Cows

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  • 1. Key Laboratory for Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. Institute of Animal Science and Veterinary, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2016-09-09

  Online published: 2017-03-09

Abstract

This experiment was conducted to study the effects of tea saponin on serum immune related cytokines contents and their mRNA expression levels of Holstein cows. Four rumen fistulated cows were used following a 4×4 Latin square design with different infusion concentrations of tea saponin[0 (control), 15, 30, 45 g/d]. The experiment had 4 periods, and each period consisted of 14 d per feeding period and 7 d sampling period. Enzyme-linked immunosorbent assay (ELISA) method kit was used to measure the contents of immunoglobulins, interleukin and tumor necrosis factor alpha (TNF-α) in serum. Quantitative real-time PCR was used to measure the mRNA expression levels of interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-10 (IL-10) and TNF-α. The results showed as follows:1) the serum immunoglobulin M (IgM) content of dairy cow in 30 g/d tea saponin group was significantly higher than that in control group (P<0.05). 2) The serum IL-1 content in 15 g/d tea saponin group was significantly higher than that in control group and 45 g/d tea saponin group (P<0.05). 3) Compared with the control group, the mRNA expression levels of IL-1, IL-10, IL-6 and TNF-α in 15, 30 and 45 g/d tea saponin groups were not significantly different (P>0.05), but the mRNA expression levels of above cytokines in 45 g/d tea saponin group was increased. In conclusion, tea saponin can improve the contents of immunoglobulins and immune related cytokines in serum, as well as the mRNA expression level of cytokine IL-6, which indicate that tea saponin can improve the immune function of dairy cows.

Cite this article

CHANG Xiaoxiao, ZHANG Yifu, ZHAO Shiping, JIANG Qihui, WANG Bing, FANG Luoyun, XIONG Benhai, JIANG Linshu . Effects of Tea Saponin on Immune Function of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2017 , 29(3) : 1039 -1045 . DOI: 10.3969/j.issn.1006-267x.2017.03.038

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