Effects of Subacute Ruminal Acidosis on Plasma Abnormal Metabolites and Biochemical Indexes of Dairy Goats

SUN Yanyong, HU Honglian, GAO Min, XU Ming, SONG Liwen

doi:10.3969/j.issn.1006-267x.2017.03.039

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 3: 1046 - 1055

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.039

Molecular Nutrition

Effects of Subacute Ruminal Acidosis on Plasma Abnormal Metabolites and Biochemical Indexes of Dairy Goats

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1. College of Animal Science and Technology, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Research Institute of Animal Nutrition, Inner Mongolia Academy of Animal Sciences, Hohhot 010030, China

Received date: 2016-09-06

Online published: 2017-03-09

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Abstract

Using the method of increasing dietary non-fiber carbohydrate/neutral detergent fiber (NFC/NDF) to induce subacute ruminal acidosis (SARA) of dairy goats for the purpose of investigating the changes of lipopolysaccharide (LPS) and histamine (HIS) contents, and biochemical indexes in plasma. Four healthy Saneng dairy goats with similar body weight were selected. Goats were fed 4 diets with different NFC/NDF (1.40, 1.79, 2.31 and 3.23, respectively) in order. Each diet was fed for 15 days as 1 group, the first 12 days were for adjustment period, and the last 3 days were for sampling period. Plasma LPS and HIS contents were detected with the method of enzyme linked immunosorbent assay; plasma biochemical indexes were detected with biochemical analyzer. The results showed as follows:1) SARA was successfully inducted when NFC/NDF reached the level of 3.23 since 1.40.2) The contents of HIS and LPS in plasma were both increased with the gradual increase of dietary NFC/NDF during SARA induction, and 3.23 NFC/NDF group was significantly higher than 1.40 NFC/NDF group (P<0.05). 3) With the increase of dietary NFC/NDF, the contents of immunoglobulin M (IgM), immunoglobulin A (IgA) and immunoglobulin G (IgG) in plasma were not significantly changed (P>0.05); the contents of creatinine (CREA) and D-lactic acid (LD) and the activities of creatine kinase (CK), aspartate aminotransferase (AST) and diamine oxidase (DAO) were not significantly changed (P>0.05); plasma γ-glutamyl transpeptidase (γ-GT) activity tended to be increased, and 3.23 NFC/NDF group was significantly higher than other groups (P<0.05); plasma urea nitrogen (UN) content was decreased, and 1.40 NFC/NDF group was significantly higher than other groups (P<0.05); plasma alkaline phosphatase (ALP) activity was increased, and 3.23 NFC/NDF group was significantly higher than other groups (P<0.01); plasma free fatty acid (FFA) content was firstly increased and then decreased, 2.31 NFC/NDF group was significantly higher than other groups (P<0.01); plasma β-hydroxybutyric acid (β-HB) content was decreased, and 1.40 NFC/NDF group was significantly higher than other groups (P<0.01). The results indicate that the contents of HIS and LPS in plasma are both significantly increased with the gradual increase of dietary NFC/NDF, which lead to inflammatory reaction in dairy goats, aggravate illness of SARA, and active immune reaction; different changes of biochemical indexes in plasma are observed, which suggests that dairy goats is in a state of stress during SARA period, and liver damage is caused.

Key words： subacute ruminal acidosis; biochemical indexes; dairy goats; non-fiber carbohydrate/neutral detergent fiber; lipopolysaccharide; histamine

Cite this article

SUN Yanyong, HU Honglian, GAO Min, XU Ming, SONG Liwen . Effects of Subacute Ruminal Acidosis on Plasma Abnormal Metabolites and Biochemical Indexes of Dairy Goats[J]. Chinese Journal of Animal Nutrition, 2017 , 29(3) : 1046 -1055 . DOI: 10.3969/j.issn.1006-267x.2017.03.039

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