Effects of Polysavone on Antioxidant Ability, Lipid Metabolism and Related Gene Expression of Laying Hens during Later Period of Laying

YANG Yu, SUN Yu, SUN Baosheng, LU Yingjie, LI Jianhui, MIAO Zhiqiang, ZHANG Junzhen

doi:10.3969/j.issn.1006-267x.2017.04.019

2017 , Vol. 29 >Issue 4: 1233 - 1240

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.04.019

Molecular Nutrition

Effects of Polysavone on Antioxidant Ability, Lipid Metabolism and Related Gene Expression of Laying Hens during Later Period of Laying

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College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China

Received date: 2016-10-19

Online published: 2017-04-14

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Abstract

This experiment was to investigate the effects of different polysavone supplemental levels on the antioxidant ability, lipid metabolism and related gene expression of laying hens during later period of laying. Totally, three hundred and twenty-four Hy-Line Brown hens at 69-week old were randomly divided into 3 groups with 6 replicates and there were 18 hens for each replication. The hens in control group were fed a basal diet, and the two others were fed the basal diet supplemented with polysavone at the levels of 500 and 1 000 mg/kg, respectively. The preliminary experiment lasted for 2 weeks and the formal experiment lasted for 8 weeks. The results showed that compared with the control group, 1) diet supplemented with polysavone significantly increased the laying rate and reduced the feed to egg ratio (P<0.05). 2) Diet supplemented with 500 mg/kg polysavone significantly increased the eggshell strength (P<0.05), significantly decreased the liver triglyceride content (P<0.01) and the cholesterol content (P<0.05). 3) Diet supplemented with 1 000 mg/kg polysavone extremely significantly increased the activities of plasma glutathion peroxidase (GSH-Px), total superoxide dismutase (T-SOD) and liver GSH-Px activity, as well as the expression quantity of thioredoxin reductase (TrxR1), cholesterol alpha-hydroxylase (CYP7A1) and sterol regulatory element binding protein-1c (SREBP-1c) (P<0.01) and the plasma total antioxidant capacity (T-AOC) (P<0.05), also significantly reduced the malondialdehyde (MDA) content in plasma (P<0.05), contents of total cholesterol and triglyceride in liver and egg yolk and the expression quantity of 3-hydroxy-3-methyl glutaric acid acyl coenzyme A reductase (HMGCR) (P<0.01). However, the addition of polysavone at the level of 1 000 mg/kg had no effects on the expression quantity of GSH-Px (P>0.05). It can be concluded that diet supplemented with polysavone can reduce the cholesterol and triglyceride content in egg yolk, increase the growth performance, eggshell strength, antioxidant and lipid metabolism capacity of Hy-Line Brown hens during late period of laying and the 1 000 mg/kg polysavone is suggested as the optimal dosage in this study.

Key words： laying hens; polysavone; antioxidant; lipid metabolism; gene expression

Cite this article

YANG Yu, SUN Yu, SUN Baosheng, LU Yingjie, LI Jianhui, MIAO Zhiqiang, ZHANG Junzhen . Effects of Polysavone on Antioxidant Ability, Lipid Metabolism and Related Gene Expression of Laying Hens during Later Period of Laying[J]. Chinese Journal of Animal Nutrition, 2017 , 29(4) : 1233 -1240 . DOI: 10.3969/j.issn.1006-267x.2017.04.019

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