Molecular Nutrition

An in Vitro Study of Umbilical Cord Mesenchymal Stem Cells Inhibite Bovine Mammary Gland Epithelial Cells Apoptosis by Insulin Like Growth Factor-Ⅰ Mediated Janus Kinase/Signal Transducer and Activator of Transcription Signaling Pathway

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  • Xinjiang Meat Emulsions with Plant-Eating Animal Nutrition Laboratory, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2016-09-28

  Online published: 2017-04-14

Abstract

This experiment was conducted to explore whether Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway was involved in regulation of umbilical cord mesenchymal stem cells (UC-MSCs) by insulin like growth factor-Ⅰ (IGF-Ⅰ) inhibited bovine mammary gland epithelial cells (BMECs) apoptosis. UC-MSCs and BMECs were co-cultured by TranswellTM double chamber, BMECs cultured alone as control, cells was treated with insulin like growth factor-Ⅰ receptor (IGF-ⅠR) inhibitor AG1024 and signal blocking agent AG490. After 24 h, the relative expression abundances of B-cell lymphoma/leukemia-2 (Bcl-2), Bcl-associated x protein (Bax), cysteine aspartic acid specific protease (Caspase-3) were detected by real-time quantitative PCR (RT-qPCR), and flow cytometry was adopted to detect apoptosis changes of cells. The results showed as follows:the apoptosis rate of BMECs in UC-MSCs and BMECs co-culture group was significantly lower than that in other groups (P<0.01); compared with BMECs group, the relative expression abundance of Bcl-2 gene in UC-MSCs and BMECs co-culture group was significantly increased (P<0.01), while the relative expression abundances of Caspase-3 and Bax genes were significantly decreased (P<0.05 or P<0.01). Treated by AG1024 or/and AG490, the apoptosis rate of single cultured BMSCs and co-cultured BMSCs with UC-MSCs was raised, and the relative expression abundances of Bax and Caspase-3 genes were up-regulated, while the relative expression abundance of Bcl-2 gene was down-regulated, and they were statistically significant (P<0.05 or P<0.01). In conclusion, UC-MSCs can regulate the expression of apoptosis relation genes in BMECs by IGF-Ⅰ mediated JAK/STAT signaling pathway, and reduce the apoptosis rate of BMECs.

Cite this article

ZHAO Yankun, SHAO Wei, LUO Chenglong, WU Kaile, YU Xiong . An in Vitro Study of Umbilical Cord Mesenchymal Stem Cells Inhibite Bovine Mammary Gland Epithelial Cells Apoptosis by Insulin Like Growth Factor-Ⅰ Mediated Janus Kinase/Signal Transducer and Activator of Transcription Signaling Pathway[J]. Chinese Journal of Animal Nutrition, 2017 , 29(4) : 1335 -1342 . DOI: 10.3969/j.issn.1006-267x.2017.04.031

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