Effects of Alfalfa Saponins on mRNA Expressions of Low-Density Lipoprotein Receptor and Adenosine Triphosphate Binding Cassette Transporters in Liver and Liver Cells of Rats

LIU Boshuai, WANG Wenjing, CHEN Yanyan, ZHU Xiaoyan, YUAN Dedi, QI Shengli, WANG Chengzhang, SHI Yinghua

doi:10.3969/j.issn.1006-267x.2017.04.043

2017 , Vol. 29 >Issue 4: 1437 - 1445

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.04.043

Molecular Nutrition

Effects of Alfalfa Saponins on mRNA Expressions of Low-Density Lipoprotein Receptor and Adenosine Triphosphate Binding Cassette Transporters in Liver and Liver Cells of Rats

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College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China

Received date: 2016-09-08

Online published: 2017-04-14

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Abstract

This experiment was conducted to investigate the effects of alfalfa saponins (AS) on mRNA expressions of low-density lipoprotein receptor (LDLR), adenosine triphosphate binding cassette transporter G5 (ABCG5) and adenosine triphosphate binding cassette transporter G8 (ABCG8) in liver and liver cells of rats, and discuss the mechanism of AS regulating cholesterol elimination and transportation at both individual and cellular levels. Hyperlipidemic rat model was set up using high fat diet, and the effects of AS on serum indexes[total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) contents] and liver LDLR, ABCG5 and ABCG8 mRNA expressions of normal and hyperlipidemic rats were determined; hyperlipidemic cell model was set up using high DMEM culture medium, the effects of AS concentration on BRL cell activity, and AS on LDLR, ABCG5 and ABCG8 mRNA expressions of normal and hyperlipidemic cells were determined. The results showed as follows:1) AS significantly decreased the contents of triglyceride, total chelesterol and low-density lipoprotein cholesterol in serum of hyperlipidemic rats (P<0.05); 2) AS significantly up-regulated mRNA expressions of LDLR, ABCG5 and ABCG8 in liver of normal rats and those of ABCG5 and ABCG8 in liver of hyperlipidemic rats (P<0.05); 3) the supplementation of 200 and 250 μg/mL AS increased BRL cell activity (P<0.05); 4) AS significantly up-regulated mRNA expressions of LDLR, ABCG5 and ABCG8 of normal BRL cells (P<0.05), however, AS had no significant effects on those of hyperlipidemic BRL cells. The results indicate that AS may accelerate the elimination and transportation of cholesterol by up-regulating mRNA expressions of LDLR, ABCG5 and ABCG8, and then reduce the content of cholesterol in body.

Key words： alfalfa saponins; rat; BRL cell; low-density lipoprotein receptor; adenosine triphosphate binding cassette transporter G5; adenosine triphosphate binding cassette transporter G8; mRNA expression

Cite this article

LIU Boshuai, WANG Wenjing, CHEN Yanyan, ZHU Xiaoyan, YUAN Dedi, QI Shengli, WANG Chengzhang, SHI Yinghua . Effects of Alfalfa Saponins on mRNA Expressions of Low-Density Lipoprotein Receptor and Adenosine Triphosphate Binding Cassette Transporters in Liver and Liver Cells of Rats[J]. Chinese Journal of Animal Nutrition, 2017 , 29(4) : 1437 -1445 . DOI: 10.3969/j.issn.1006-267x.2017.04.043

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