Molecular Nutrition

A Study on Intestinal Microbiota Diversity of 3- to 6-Month-Old Yili Horses

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  • Xinjiang Key Laboratory of Herbivore Nutrition for Meat & Milk Production, Xingjiang Agricultural University, Urumqi 830052, China

Received date: 2016-11-11

  Online published: 2017-05-13

Abstract

The current study was conducted to evaluate the intestinal microbiota diversity of 3- to 6-month-old Yili horses, to reveal the regulation of succession of intestinal micrbiota before weaning, and to provide a theoretical basis for the researches of growth and intestinal health of young horse. Five 3-month-old Yili horses with the average body weight of (89.75±8.81) kg and the same birth day were selected to conduct a 90-day feeding trial. The feces samples were collected on days 0 (1 day before the experiment), 30, 60 and 90 of the experiment, respectively. The microbial genomic total DNA was extracted from each feces sample. Microbiota diversity of feces was evaluated using Illumina HiSeq sequencing technology. The results showed as follows: 1) twenty feces samples for 5 colts were sequenced, a total of 157 665 effective sequences and 1 117 average operational taxonomic units (OTUs) were acquired. 2) The alpha diversity indexes (ACE, Chao1, Shannon and Simpson indices) of feces microbiota showed fluctuated changes with the age increasing, but the changes were not significant among different time points (P>0.05). 3) At the phylum level, ten dominant bacteria in feces of colts were Firmicutes, Bacteroidetes, Proteobacteria, Verrucomicrobia, Spirochaetes, Fusobacteria, Tenericutes, Actinobacteria, TM7 and Euryarchaeota, and the abundances of Firmicutes, Bacteroidetes, Proteobacteria and Verrucomicrobia were higher. At the family level, ten dominant bacteria in feces of colts were Bacillaceae, Moraxellaceae, Planococcaceae, Carnobacteriaceae, BS11, RFP12, Lactobacillaceae, Ruminococcaceae, Lachnospiraceae and Porphyromonadaceae. At the genera level, ten dominant bacteria in feces of colts were Acinetobacter, Desemzia, Lactobacillus, Ureibacillus, Paludibacter, Bacillus, Escherichia, Carnobacterium, Treponema and Mogibacterium. Base on the results, it is concluded that the intestinal microbiota of 3- to 6-month-old Yili horses can be accurately classified using Illumina HiSeq sequencing technology. The Firmicutes, Bacteroidetes, Proteobacteria, Verrucomicrobia were the dominant bacteria in intestine of 3- to 6-month-old Yili horses.

Cite this article

LI Xiaobin, ZHAO Guodong, LIU Zhen, WU Tingting, HE Zhourui, DENG Haifeng, YANG Kailun . A Study on Intestinal Microbiota Diversity of 3- to 6-Month-Old Yili Horses[J]. Chinese Journal of Animal Nutrition, 2017 , 29(5) : 1535 -1544 . DOI: 10.3969/j.issn.1006-267x.2017.05.011

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