A Study on Differential Proteomics of Chicks Serum before and after Cold Stress

YANG Yuying, LIU Peng, HE Jun, LIU Yang, YAO Ruizhi, SHI Hongzhao, ZHEN Li, TANG Dejiang, LI Shize

doi:10.3969/j.issn.1006-267x.2017.05.015

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 5: 1567 - 1573

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.05.015

Molecular Nutrition

A Study on Differential Proteomics of Chicks Serum before and after Cold Stress

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College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2016-11-11

Online published: 2017-05-13

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Abstract

The objective of this experiment was to compare and analysis the difference of protein expression in serum of chicks before and after cold stress, and to identify the differential proteins. Thirty chicks were randomly divided into three groups, which were cold stress group, cold-adapted group and normal temperature control group. Blood samples of chicks in each group were collected and prepared the serum for two-dimensional gel electrophoresis (2-DE), in order to obtain the 2-DE maps for protein expressions in serum. The differences of 2-DE maps were analysed by PDQuest 8.0 software. After chicks' serum differential expression proteins were analysed, matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) technology was used to identify the differential expression proteins, and the differential expression proteins were verified by Western blot method. The results revealed that a relatively complete differential protein data for chicks' serum in normal temperature control group, cold stress group and cold-adapted group were obtained by 2-DE analysis, and 23 differential protein spots were detected. MALDI-TOF-MS technology was utilized to analyze several of reproducible and more obvious protein spots, successfully identifying 4 different proteins, two of them were aldolase C fructose-bisphosphate (ALDOC), which was related protein involved in glucose and energy metabolism pathway. Then, by using the Western blot method for further validated differential protein ALDOC, the results were consistent with the results of the 2-DE. Therefore, the results indicated that the expressions of proteins in serum of chicks before and after cold stress are significantly different. The expression differences of these proteins may relate to cold stress.

Key words： two-dimensional electrophoresis; cold stress; proteome; differential expression protein; differential protein spots

Cite this article

YANG Yuying, LIU Peng, HE Jun, LIU Yang, YAO Ruizhi, SHI Hongzhao, ZHEN Li, TANG Dejiang, LI Shize . A Study on Differential Proteomics of Chicks Serum before and after Cold Stress[J]. Chinese Journal of Animal Nutrition, 2017 , 29(5) : 1567 -1573 . DOI: 10.3969/j.issn.1006-267x.2017.05.015

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