Molecular Nutrition

Ruminal Ciliate Community Structure of Chuanzhong Black Goats: An Analysis Using High-Throughput Sequencing Technology

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  • Key Laboratory of Animal Disease and Human Health of Sichuan Province, Key Laboratory of Environmental Hazard and Animal Disease of Sichuan Province, College of Veterinary Medicine, Sichuang Agricultural University, Chengdu 611130, China

Received date: 2016-11-04

  Online published: 2017-05-13

Abstract

This study was designed to reveal the ruminal ciliate community structure of Chuanzhong black goats using MiSeq sequencing technology. Three 4-month-old Chuanzhong black goats weighted (15.53±0.21) kg were used to collect rumen fluid, and the samples were collected after normal feeding of 20 (sample A) and 60 d (sample F), respectively. Total DNA was extracted for amplification V4 area of 18S rRNA, and the products were sequenced by Illumina MiSeq sequencing system. The results showed as follows: 1) a total of 242 321 high quality valid sequences and 1 650 operational taxonomic units were obtained. 2) There were no significant difference on alpha diversity indexes (Chao, ACE, Shannon and Simpson) between samples A and F (P>0.05). 3) At class level, the most abundant class in samples A and F were Ciliophora, Litostomatea (sample A was 46.0%, sample F was 44.7%), and there was no significant difference between samples A and F (P>0.05). 4) At family level, the most abundant family in sample A was Ophryoscolecidae (31.8%) followed by Isotrichidae (14.2%); the most abundant family in sample F was Ophryoscolecidae (42.8%); the relative abundance of Ophryoscolecidae in sample F was significantly higher than that in sample A (P<0.05), and the relative abundance of Isotrichidae in sample A was significantly higher than that in sample F (P<0.05). 5) At genus level, the most abundant ciliate genus was Polyplastron(sample A was 20.9%, sample F was 25.4%), and there are no significant differences between samples A and F (P>0.05); there were significant differences of the relative abundances of Isotricha, Ophryoscolex, Diploplastron and Enoploplastron, the relative abundances of Isotricha (14.1% vs. 1.9%) and Diploplastron (2.8% vs. 1.5%) in sample A were significantly higher than those in sample F (P<0.05), and relative abundances of Ophryoscolex (6.7% vs. 12.5%) and Enoploplastron (0.3% vs. 2.5%) in sample A were significantly lower than those in sample F (P<0.05). In conclusion, Polyplastron is the most abundant genus in the rumen ciliate communities of youth Chuanzhong black goats, and there are still a lot of unclassified and unidentified eukaryotic microorganisms with high abundance worth further study in rumen.

Cite this article

LIU Qi, CHEN Yun, DENG Junliang, REN Zhihua, YANG Yanyi, GAO Shuang, CHEN Chong . Ruminal Ciliate Community Structure of Chuanzhong Black Goats: An Analysis Using High-Throughput Sequencing Technology[J]. Chinese Journal of Animal Nutrition, 2017 , 29(5) : 1574 -1581 . DOI: 10.3969/j.issn.1006-267x.2017.05.016

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