Effects of Betulinic Acid on Serum Parameters of Oxidative Stressed Mice Induced by Dexamethasone

ZHU Lijuan, ZHAO Jing, XIANG Siting, WANG Xihong, HUANG Lin, YI Xianglian, ZHU Ruocen, WU Jing, YI Jin

doi:10.3969/j.issn.1006-267x.2017.05.022

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 5: 1627 - 1633

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.05.022

Molecular Nutrition

Effects of Betulinic Acid on Serum Parameters of Oxidative Stressed Mice Induced by Dexamethasone

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1. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China

Received date: 2016-10-31

Online published: 2017-05-13

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Abstract

The objective of this research is to evaluate the effects of betulinic acid (BA) on serum parameters of oxidative stressed mice induced by dexamethasone (Dex). Forty male Kunming mice were randomly divided into 5 groups: control (NC) group, Dex group, 0.25 mg/kg BA group, 0.50 mg/kg BA group and 1.00 mg/kg BA group, each group had 8 mice. Mice in control group and Dex group were orally administered 1% starch solution and the other groups were orally administered different doses of BA for 14 days. Except NC group, mice in the other groups were intraperitoneal injected Dex at dosage of 25 mg/kg to set up oxidative damage model. After 15 hours, blood was collected from eyes to prepare serum. The activities of alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the contents of total protein (TP), albumin (ALB), total cholesterol (TC), triglyceride (TG) and total bilirubin (T-Bil), calcium ions (Ca²⁺) and cytochrome c (CytC), total antioxidant capacity (T-AOC), inhabitation capacity of hydroxyl free radical, superoxide dismutase (SOD) activity, and the contents of glutathione(GSH) and malondialdehyde (MDA) were determined. The results showed as follows: BA pretreatment could significantly reduce serum activity of ALP and contents of TG, T-Bil and CytC of oxidative stressed mice (P<0.01 or P<0.05), and the effects were dose depended; BA pretreatment could significantly decrease serum content of MDA in low dose (0.25 and 0.50 mg/kg) BA groups (P<0.05 or P<0.01), while could significantly increase the content in high dose (1.00 mg/kg) BA group (P<0.01); BA pretreatment could significantly increase serum Ca²⁺ and GSH contents, SOD activity, T-AOC and inhibition capacity of hydroxyl free radical (P<0.05 or P<0.01), and the effects were dose depended; BA pretreatment could decrease serum ALT and AST activities, but the effects were not significant (P>0.05), and serum contents of TP and ALB were not significantly changed (P>0.05). The results suggest that BA can effectively improve serum parameters of mice and shows preventive protection of oxidative stress induced by Dex.

Key words： betulinic acid; dexamethasone; oxidative stress; serum parameters

Cite this article

ZHU Lijuan, ZHAO Jing, XIANG Siting, WANG Xihong, HUANG Lin, YI Xianglian, ZHU Ruocen, WU Jing, YI Jin . Effects of Betulinic Acid on Serum Parameters of Oxidative Stressed Mice Induced by Dexamethasone[J]. Chinese Journal of Animal Nutrition, 2017 , 29(5) : 1627 -1633 . DOI: 10.3969/j.issn.1006-267x.2017.05.022

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