Effects of Oil Types on Glyceraldehyde-3-Phosphate Dehydrogenase Gene Relative Expression Level in Different Tissues of Broilers

TAO Yafei, CHEN Qing, XU Yuanyuan, SHI Xiuwen, CHEN Wen, HUANG Yanqun

doi:10.3969/j.issn.1006-267x.2017.05.035

2017 , Vol. 29 >Issue 5: 1750 - 1758

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.05.035

Molecular Nutrition

Effects of Oil Types on Glyceraldehyde-3-Phosphate Dehydrogenase Gene Relative Expression Level in Different Tissues of Broilers

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Henan Agricultural University National Germplasm Resoueces Platform for Animals, Feed Nutrition Engineering Laboratory of Henan Province, Henan Agricultural University, Zhengzhou 450002, China

Received date: 2016-11-17

Online published: 2017-05-13

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Abstract

This experiment was conducted to study the effects of oil types on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene relative expression level in different tissues of broilers. A total of 240 one-day-old female Cobb broilers were selected and randomly assigned to 8 groups (four single oil groups were fed diets added with 5.00% linseed oil, corn oil, sesame oil and lard oil, respectively; four oil combination groups were fed diets added with 2.50% lard oil+2.50% corn oil, 2.50% lard oil+2.50% sesame oil, 2.50% linseed oil+2.50% corn oil and 2.50% linseed oil+2.50% sesame oil, respectively) with 6 replicates per group and 5 broilers per replicate. The experiment lasted for 42 days. The results showed as follows: 1) the relative expression level of GAPDH gene in broiler tissues was significantly affected by tissues and the interaction between oil types and tissues (P<0.05), however, the effects of oil types were not significant (P>0.05). The relative expression level in pectorals of GAPDH gene of 42-day-old broilers was significantly higher than that in liver and abdominal fat (P<0.05), the relative expression level of GAPDH gene in liver was 37.50 to 89.50 and 129.54 to 190.64 times than that in liver and abdominal fat, respectively, however, the relative expression level of GAPDH gene between liver and abdominal fat was not significant (P>0.05). The relative expression level of GAPDH gene in corn oil group was significantly higher than that in sesame oil group in pectorals (P<0.05). 2) The relative expression level of GAPDH gene in liver of 21-day-old broilers was significantly higher than that of 42-day-old broilers (P<0.05 or P<0.01). 3) The relative expression level of GAPDH gene in liver of broilers was significantly affected by days of age (P<0.05), however, the effects of oil combination and the interaction between oil combination and days of age were not significant (P>0.05). In conclusion, the effect of oil types on the relative expression level of GAPDH gene in broilers shows difference among different tissues, corn oil can improve the relative expression level of GAPDH gene in pectorals. The relative expression level in pectorals of GAPDH gene of 42-day-old broilers is significantly higher than that in liver and abdominal fat, and the relative expression level of GAPDH gene in liver of 21-day-old broilers is significantly higher than that of 42-day-old broilers.

Key words： broilers; oil; GAPDH; gene expression

Cite this article

TAO Yafei, CHEN Qing, XU Yuanyuan, SHI Xiuwen, CHEN Wen, HUANG Yanqun . Effects of Oil Types on Glyceraldehyde-3-Phosphate Dehydrogenase Gene Relative Expression Level in Different Tissues of Broilers[J]. Chinese Journal of Animal Nutrition, 2017 , 29(5) : 1750 -1758 . DOI: 10.3969/j.issn.1006-267x.2017.05.035

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