Effects of Rumen-Protected Methionine on Ruminal Microbial Protein Production, Milk Performance and Nitrogen Excretion of Dairy Cows

ZHANG Chengxi, SUN Youde, LIU Xiwu, SUN Guoqiang

doi:10.3969/j.issn.1006-267x.2017.05.036

2017 , Vol. 29 >Issue 5: 1759 - 1766

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.05.036

Molecular Nutrition

Effects of Rumen-Protected Methionine on Ruminal Microbial Protein Production, Milk Performance and Nitrogen Excretion of Dairy Cows

Expand

1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China;
2. Institute of Animal Husbandry and Veterinary Medicine of Qingdao, Qingdao 266100, China

Received date: 2016-11-01

Online published: 2017-05-13

Fold

Abstract

This experiment was conducted to determine the effects of rumen-protected methionine (RPMet) on ruminal microbial protein (MCP) production, milk performance and nitrogen excretion of dairy cows. Forty Holstein lactating cows with similar age, body weight, parity, milk yield, milk composition and lactation period [(90±15) d] were randomly divided into 4 groups with 10 cows per group. The supplement level of RPMet in control and test groups 1, 2 and 3 was 0, 15, 25 and 35 g/(d·head), respectively. The pretest lasted for 15 d, and the test lasted for 60 d. The results showed as follows: 1) ruminal MCP production in test groups 1, 2 and 3 was extremely significantly increased by 13.10%, 20.45% and 16.23% compared with control group (P<0.01), respectively. 2) Milk yield in test groups 1, 2 and 3 was significantly increased by 8.12%, 13.32% and 10.32% compared with control group (P<0.05 or P<0.01), respectively; RPMet could significantly increase milk fat percentage and milk protein percentage (P<0.05 or P<0.01), and could significantly reduce milk somatic cell count (P<0.05 or P<0.01), especially test group 2 was the lowest. 3) Compared with control group, total nitrogen excretion in test groups was extremely significantly reduced (P<0.01), and was reduced by 8.55%, 17.49% and 13.25% in test groups 1, 2 and 3, respectively. It can be seen that the addition of RPMet to diets can significantly increase ruminal MCP production, decrease nitrogen excretion, and improve the performance of dairy cows under the present experimental conditions. The optimal dosage of RPMet was 25 g/(d·head).

Key words： rumen-protected methionine; ruminal microbial protein; milk performance; nitrogen excretion

Cite this article

ZHANG Chengxi, SUN Youde, LIU Xiwu, SUN Guoqiang . Effects of Rumen-Protected Methionine on Ruminal Microbial Protein Production, Milk Performance and Nitrogen Excretion of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2017 , 29(5) : 1759 -1766 . DOI: 10.3969/j.issn.1006-267x.2017.05.036

References

[1] 毛成文.包被蛋氨酸和赖氨酸对肉羊氮代谢和生产性能影响研究[D].硕士学位论文.北京:中国农业大学,2004.

[2] 贾文彬,李建国,赵世芳.反刍动物过瘤胃氨基酸的研究进展[J].饲料博览,2005(12):10-12.

[3] 董贤文.过瘤胃赖氨酸、过瘤胃蛋氨酸的研发[D].硕士学位论文.重庆:西南大学,2013.

[4] 韩兆玉,王根林.奶牛过瘤胃蛋氨酸营养研究进展[J].饲料研究,2006(2):33-35.

[5] 邹阿玲,孙国军,李明强,等.过瘤胃蛋氨酸对泌乳早期奶牛生产性能的影响[J].中国奶牛,2005(2):27-29.

[6] 燕磊.瘤胃保护性蛋氨酸对小尾寒羊氨基酸代谢影响的研究[D].硕士学位论文.泰安:山东农业大学,2005.

[7] SALSBURY R L,MARVIL D K,WOODMANSEE C W,et al.Utilization of methionine and methionine hydroxy analog by rumen microorganisms in vitro[J].Journal of Dairy Science,1971,54(3):390-396.

[8] 韩占强,林英庭,赵发盛,等.不同过瘤胃蛋氨酸对奶牛pH和氨态氮的影响[J].饲料研究,2008(7):52-55.

[9] 冯仰廉,陆治年.奶牛营养需要和饲料成分[M].3版.北京:中国农业出版社,2007:2.

[10] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007:49-74.

[11] 朱雯.粗料来源对奶牛乳蛋白前体物生成与生产性能的影响与机制研究[D].博士学位论文.杭州:浙江大学,2013:39-69.

[12] CHEN X B,MATUSZEWSKI W,KOWALCZYK J.Determination of allantoin in biological,cosmetic,and pharmaceutical samples[J].Journal of AOAC International,1996,79(3):628-635.

[13] KOHN R A,FRENCH K R,RUSSEK-COHEN E.A comparison of instruments and laboratories used to measure milk urea nitrogen in bulk-tank milk samples[J].Journal of Dairy Science,2004,87(6):1848-1853.

[14] VALADARES R F D,BRODERICK G A,FILHO S C V,et al.Effect of replacing alfalfa silage with high moisture corn on ruminal protein synthesis estimated from excretion of total purine derivatives[J].Journal of Dairy Science,1999,82(12):2686-2696.

[15] 武安泉,杨开伦,雒秋江,等.瘤胃投注N-乙酰-DL-蛋氨酸对绵羊瘤胃消化代谢的影响[J].草食家畜,2006(2):39-44.

[16] 邹彩霞,唐庆凤,李舒露,等.饲粮添加过瘤胃赖氨酸和过瘤胃蛋氨酸对泌乳水牛生产性能的影响[J].动物营养学报,2010,22(5):1445-1450.

[17] 杨维仁.瘤胃保护性氨基酸对肉牛消化代谢影响及适宜供给量的研究[D].博士学位论文.北京:中国农业大学,2004.

[18] BERTHIAUME R,LAPIERRE H,STEVENSON M,et al.Comparison of the in situ and in vivo intestinal disappearance of ruminally protected methionine[J].Journal of Dairy Science,2000,83(9):2049-2056.

[19] 高红建.氮-羟甲基蛋氨酸钙对绵羊瘤胃代谢及营养物质消化代谢的影响[D].硕士学位论文.北京:中国农业科学院,2002.

[20] 段红伟.N-羟甲基蛋氨酸钙的过瘤胃效果及其对瘤胃环境和饲料养分消化的影响[D].硕士学位论文.兰州:甘肃农业大学,2000.

[21] 熊春梅.氮-羟甲基蛋氨酸钙对中国荷斯坦奶牛瘤胃代谢及生产性能的影响[D].硕士学位论文.兰州:甘肃农业大学,2004.

[22] 韩兆玉,周国波,金志红,等.过瘤胃蛋氨酸对热应激下奶牛生产性能、淋巴细胞凋亡以及相关基因的影响[J].动物营养学报,2009,21(5):665-672.

[23] 姜明鑫,杨连玉.过瘤胃胆碱和蛋氨酸对泌乳早期奶牛生产性能和健康状况影响的研究现状[J].经济动物学报,2013,17(4):228-231,235.

[24] 毕晓华,张晓明.过瘤胃保护蛋氨酸对奶牛氨基酸代谢和血液生化指标的影响[J].饲料研究,2014(21):48-53.

[25] 谢实勇,贾志海,朱晓萍,等.包被蛋氨酸对内蒙古白绒山羊氮代谢及产绒性能的影响[J].中国农业大学学报,2003,8(3):73-76.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References