Effects of Methionine Level on Development of Body Condition, Morphology of Digestive Tract and Serum Antioxidant Indexes in Lambs

WANG Jie, CUI Kai, WANG Shiqin, DIAO Qiyu, ZHANG Naifeng

doi:10.3969/j.issn.1006-267x.2017.05.040

2017 , Vol. 29 >Issue 5: 1792 - 1802

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.05.040

Molecular Nutrition

Effects of Methionine Level on Development of Body Condition, Morphology of Digestive Tract and Serum Antioxidant Indexes in Lambs

Expand

Feed Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Beijing 100081, China

Received date: 2016-11-01

Online published: 2017-05-13

Fold

Abstract

The aim of this study was to assess the effects of methionine level on development of body condition, morphology of digestive tract and serum antioxidant indexes in lambs. Twelve pairs of male Hu twin lambs weaned at 7 days of age were selected and divided into two groups [control (CON) group and low methionine level (LM) group] with a matched-pairs design, and one pair of lambs were assigned to the different groups. The experiment consisted of two stages. Stage 1 (8 to 56 days of age), lambs in the CON group were fed basal milk replacer and basal starter, while those in the LM group were fed basal milk replacer and basal starter deducting 0.70% and 0.40% methionine on the basis of CON group, respectively. Stage 2 (57 to 84 days of age), all lambs were stop feeding milk replacer and fed basal starter. Six twins at 56 and 84 days of age were slaughtered to collect gastrointestinal tract tissues and serum sample. The results showed as follows: 1) for feed intake of methionine, LM group was significantly lower than CON group at 8 to 56 days of age and 8 to 84 days of age (P<0.01), but dry matter intake in LM group was significantly higher than that in CON group (P<0.05); at 56 days of age, no significant differences were observed in development of body condition indexes between groups (P>0.05) except that body weight, body length, heart girth, body length index in LM group were significantly lower than those in CON group (P<0.05); at 84 days of age, no significant differences were observed in development of body condition indexes between groups (P>0.05). 2) At 56 days of age, ruminal papillary width in LM group was significantly lower than that in CON group (P<0.05), but no significant differences were observed in indexes of morphology of the digestive tract between groups (P>0.05). 3) At 56 days of age, serum superoxide dismutase activity in LM group was significantly lower than that in CON group (P<0.01); at 84 days of age, no significant differences were observed in serum antioxidant indexes between groups (P>0.05) except that glutathione peroxidase activity in LM group was significantly lower than that in CON group (P<0.05). In conclusion, low methionine level of diet not only results in inhibiting the growth of body weight, body length, heart girth, body length index and ruminal papillary width, but also decreases serum superoxide dismutase activity, however, increases dry matter intake; after the improvement of dietary methionine level, a subsequent recovery appear in development of body condition and morphology development of digestive tract, but body's antioxidant system is not completely improved.

Key words： methionine; lamb; development of body condition; morphology of digestive tract; antioxidant indexes

Cite this article

WANG Jie, CUI Kai, WANG Shiqin, DIAO Qiyu, ZHANG Naifeng . Effects of Methionine Level on Development of Body Condition, Morphology of Digestive Tract and Serum Antioxidant Indexes in Lambs[J]. Chinese Journal of Animal Nutrition, 2017 , 29(5) : 1792 -1802 . DOI: 10.3969/j.issn.1006-267x.2017.05.040

References

[1] NAPOLITANO F,CIFUNI G F,PACELLI C,et al.Effect of artificial rearing on lamb welfare and meat quality[J].Meat Science,2002,60(3):307-315.

[2] 范志影,刁其玉,蔡健森.不同蛋白质来源代乳粉对羔羊肌肉化学成分的影响[J].中国畜牧杂志,2008,44(7):29-30,48.

[3] ZHAO J,HARPER M J,ESTIENNE M J,et al.Growth performance and intestinal morphology responses in early weaned pigs to supplementation of antibiotic-free diets with an organic copper complex and spray-dried plasma protein in sanitary and nonsanitary environments[J].Journal of Animal Science,2007,85(5):1302-1310.

[4] CORL B A,HARRELL R J,MOON H K,et al.Effect of animal plasma proteins on intestinal damage and recovery of neonatal pigs infected with rotavirus[J].The Journal of Nutritional Biochemistry,2007,18(12):778-784.

[5] 张庆丽.早期能量与蛋白限制饲养对1月龄断奶羔羊胃肠道发育的影响[D].硕士学位论文.杨凌:西北农林科技大学,2010.

[6] YI G F,ALLEE G L,KNIGHT C D,et al.Impact of glutamine and oasis hatchling supplement on growth performance,small intestinal morphology,and immune response of broilers vaccinated and challenged with Eimeria maxima[J].Poultry Science,2005,84(2):283-293.

[7] UNI Z,GAL-GARBER O,GEYRA A,et al.Changes in growth and function of chick small intestine epithelium due to early thermal conditioning[J].Poultry Science,2001,80(4):438-445.

[8] LIU P,PIAO X S,KIM S W,et al.Effects of chito-oligosaccharide supplementation on the growth performance,nutrient digestibility,intestinal morphology,and fecal shedding of Escherichia coli and Lactobacillus in weaning pigs[J].Journal of Animal Science,2008,86(10):2609-2618.

[9] PLUSKE J R.Morphological and functional changes in the small intestine of the newly-weaned pig[C]//PIVA A,BACH K K E,LINDBERG J E.Gut environment of pigs.Nottingham:Nottingham University Press,2001:1-27.

[10] MOURÃO J L,PINHEIRO V,ALVES A,et al.Effect of mannan oligosaccharides on the performance,intestinal morphology and cecal fermentation of fattening rabbits[J].Animal Feed Science and Technology,2006,126(1/2):107-120.

[11] ABDELRAHMAN M M,HUNAITI D A.The effect of dietary yeast and protected methionine on performance and trace minerals status of growing Awassi lambs[J].Livestock Science,2008,115(2/3):235-241.

[12] 祁敏丽,马铁伟,刁其玉,等.饲粮营养限制对断奶湖羊羔羊生长、屠宰性能以及器官发育的影响[J].畜牧兽医学报,2016,47(8):1601-1609.

[13] MANZOOR R,KAMAL T,KHWAJA D A,et al.Effects of feed accessing time after hatching on the methionine requirements,performances and digestive system development of broiler chicks[J].European Neuropsychopharmacology,2009,8(98):29-34.

[14] KRUTTHAI N,VAJRABUKKA C,MARKVICHITR K,et al.Effect of source of methionine in broken rice-soybean diet on production performance,blood chemistry,and fermentation characteristics in weaned pigs[J].Czech Journal of Animal Science,2015,60(3):123-131.

[15] EL-TAHAWY A S,ISMAEIL A M.Methionine-supplemented diet increases the general performance and value of rahmani lambs[J].Iranian Journal of Applied Animal Science,2013,3(3):513-520.

[16] 中华人民共和国农业部.NY/T 816-2004肉羊饲养标准[S].北京:中国农业出版社,2004.

[17] 刁其玉,屠焰.一种犊牛羔羊用代乳粉:中国,CN1494832A[P].2004-05-12.

[18] PATUREAU-MIRAND P,THERIEZ M.Amino acid requirements of preruminant lambs[J].Annales de Zootechnie,1977,26(2):287.

[19] 王波,柴建民,王海超,等.蛋白质水平对湖羊双胞胎公羔生长发育及肉品质的影响[J].动物营养学报,2015,27(9):2724-2735.

[20] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社,2003.

[21] 许贵善,刁其玉,纪守坤,等.不同饲喂水平对肉用绵羊生长性能、屠宰性能及器官指数的影响[J].动物营养学报,2012,24(5):953-960.

[22] 李辉.蛋白水平与来源对早期断奶犊牛消化代谢及胃肠道结构的影响[D].博士学位论文.北京:中国农业科学院,2008.

[23] 王杰,崔凯,毕研亮,等.蛋氨酸限制与补偿对羔羊生长性能及内脏器官发育的影响[J].动物营养学报,2016,28(11):3669-3678.

[24] CAROLINE B T,BARBARA S,BURRIN D G.Intestinal metabolism of sulfur amino acids[J].Nutrition Research Reviews,2009,22(2):175-187.

[25] ALBERT W W,GARRIGUS U S,FORBES R M,et al.The sulfur requirement of growing-fattening lambs in terms of methionine,sodium sulfate,and elemental sulfur[J].Journal of Animal Science,1956,15(2):559-569.

[26] GÓMEZ-PASTÉN M,MORA O,PEDRAZA-CHAVERRI J,et al.The effect of a long term feed restriction on metabolism and tissue composition of goats[J].The Journal of Agricultural Science,1999,132(2):227-232.

[27] ROOKE J A,HOUDIJK J G M,MCILVANEY K,et al.Differential effects of maternal undernutrition between days 1 and 90 of pregnancy on ewe and lamb performance and lamb parasitism in hill or lowland breeds[J].Journal of Animal Science,2010,88(12):3833-3842.

[28] GAO F,HOU X Z,LIU Y C,et al.Effect of maternal under-nutrition during late pregnancy on lamb birth weight[J].Asian-Australasian Journal of Animal Sciences,2008,21(3):371-375.

[29] PUCHALA R,PATRA A K,ANIMUT G,et al.Effects of feed restriction and realimentation on mohair fiber growth and tissue gain by growing Angora goats[J].Livestock Science,2011,138(1/2/3):180-186.

[30] 刘小刚.营养限制及补偿对羔羊内脏器官和血液中CD4⁺、CD8⁺ T淋巴细胞的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2010.

[31] 刑振全,杨红,王春微.浅谈我国奶牛体型线性鉴定方法[J].黑龙江动物繁殖,2007,15(1):28-29.

[32] 赵恒波,田黛君,罗海玲,等.纤维素复合酶对羔羊早期生长发育的影响[J].中国畜牧杂志,2007,43(23):34-37.

[33] 马存寿,冯生青,周玉青.青海高原半细毛羊羔羊断奶体重与体尺的通径分析[J].中国草食动物,2008,28(5):44-45.

[34] 陈碧红,刘庆华.戴云山羊体尺与体重的回归分析[J].中国草食动物,2011,31(5):34-36.

[35] 陈月丽,任凤芸,梁辛,等.11-14月龄奶水牛体重与体尺指标的相关性分析[J].中国畜牧兽医,2016,43(3):662-667.

[36] FERRARIS R P,CAREY H V.Intestinal transport during fasting and malnutrition[J].Annual Review of Nutrition,2000,20:195-219.

[37] LONG N M,PRADO-COOPER M J,KREHBIEL C R,et al.Effects of nutrient restriction of bovine dams during early gestation on postnatal growth,carcass and organ characteristics,and gene expression in adipose tissue and muscle[J].Journal of Animal Science,2010,88(10):3251-3261.

[38] RAMALINGAM A,WANG X X,GABELLO M,et al.Dietary methionine restriction improves colon tight junction barrier function and alters claudin expression pattern[J].American Journal of Physiology:Cell Physiology,2010,299(5):C1028-C1035.

[39] RIEDIJK M A,STOLL B,CHACKO S,et al.Methionine transmethylation and transsulfuration in the piglet gastrointestinal tract[J].Proceedings of the National Academy of Sciences of the United States of America,2007,104(9):3408-3413.

[40] MALIK G,HOEHLER D,RADEMACHER M,et al.Apparent absorption of methionine and 2-hydroxy-4-methylthiobutanoic acid from gastrointestinal tract of conventional and gnotobiotic pigs[J].Animal,2009,3(10):1378-1386.

[41] 韩正康,陈杰.反刍动物瘤胃的消化和代谢[M].北京:科学出版社,1988.

[42] SUÁREZ B J,VAN REENEN C G,STOCKHOFE N,et al.Effect of roughage source and roughage to concentrate ratio on animal performance and rumen development in veal calves[J].Journal of Dairy Science,2007,90(5):2390-2403.

[43] LESMEISTER K E,TOZER P R,HEINRICHS A J.Development and analysis of a rumen tissue sampling procedure[J].Journal of Dairy Science,2004,87(5):1336-1344.

[44] ZITNAN R,KUHLA S,NVRNBERG K,et al.Influence of the diet on the morphology of ruminal and intestinal mucosa and on intestinal carbohydrase levels in cattle[J].Veterinarni Medicina,2003,48(7):177-182.

[45] GRAHAM C,SIMMONS N L.Functional organization of the bovine rumen epithelium[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2005,288(1):R173-R181.

[46] 蔡健森.蛋白来源对早期断奶羔羊生产性能和器官发育及血清生化指标的影响[D].硕士学位论文.北京:中国农业科学院,2007.

[47] 孙志洪.关键营养素限制对断奶羔羊胃肠道发育的程序化抑制及营养干预研究[D].硕士学位论文.北京:中国科学院研究生院,2010.

[48] 聂立欣,于博.猪不同肠段肠绒毛与肠腺形态的测量研究[J].畜牧兽医科技信息,2010(6):28-29.

[49] VAREL V H,ROBINSON I M,POND W G.Effect of dietary copper sulfate,Aureo SP250,or clinoptilolite on ureolytic bacteria found in the pig large intestine[J].Applied and Environment Microbiology,1987,53(9):2009-2012.

[50] HAMPSON D J,KIDDER D E.Influence of creep feeding and weaning on brush border enzyme activities in the piglet small intestine[J].Research in Veterinary Science,1986,40(1):24-31.

[51] 顾宪红.断奶仔猪日粮蛋白质需要量及低蛋白日粮对仔猪的影响[J].中国农业科技导报,2001,3(1):34-37.

[52] 霍湘,王安利,杨建梅.含硫氨基酸的抗氧化作用[J].生物学通报,2006,41(4):3-4.

[53] 刘文斐,刘伟龙,占秀安,等.不同形式蛋氨酸对肉种鸡生产性能、免疫指标及抗氧化功能的影响[J].动物营养学报,2013,25(9):2118-2125.

[54] 马森.谷胱甘肽过氧化物酶和谷胱甘肽转硫酶研究进展[J].动物医学进展,2008,29(10):53-56.

[55] SHOVELLER A K,STOLL B,BALL R O,et al.Nutritional and functional importance of intestinal sulfur amino acid metabolism[J].The Journal of Nutrition,2005,135(7):1609-1612.

[56] 王增敏,谭利伟,尹兆正.蛋氨酸对开产蛋鸡脂肪代谢性能的影响及机理研究[J].上海畜牧兽医通讯,2009(1):4-5.

[57] 张景飞,王晓蓉,沈骅.低浓度2,4-DCP对鲫鱼肝脏抗氧化防御系统的影响[J].中国环境科学,2003,23(5):531-534.

[58] 刘秀丽,许立庆,曾宪惠,等.低硒、低蛋氨酸对大鼠体内GSH-Px及TBA值的影响[J].哈尔滨医科大学学报,1993(1):31-33.

[59] 麻丽坤,谭利伟,卫振,等.日粮蛋氨酸水平对开产蛋鸡体组织生长和产蛋性能的影响[J].浙江农业科学,2006(5):586-589.

[60] 林祯平,冯凯玲,叶慧,等.饲粮蛋氨酸水平对28~70日龄狮头鹅血清生化指标及抗氧化功能的影响[J].动物营养学报,2012,24(11):2126-2132.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References