Molecular Nutrition

Tolerance of Selenium-Yeast in Diets of Largemouth Bass (Micropterus salmoides)

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  • 1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;
    2. National Aquafeed Safety Assessment Station, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2016-12-07

  Online published: 2017-06-07

Abstract

A 10-week growth trail was conducted to evaluate the tolerance of selenium (Se)-yeast in diets of largemouth bass (Micropterus salmoides) by studying the effects of Se-yeast on growth performance, plasma biochemical indices, tissue antioxidant indices and hepatic histology of largemouth bass. Four experimental diets were prepared with Se-yeast supplemental levels at 0 (Y0), 0.5 (Y0.5), 2.5 (Y2.5) and 5.0 mg/kg (calculated in Se) (Y5.0) based on a basal diet, in which 0.5 mg/kg was designed as the maximum recommended level, and the 2.5 and 5.0 mg/kg were 5 and 10 folds of the maximum recommended level, respectively. The background Se content in the basal diet was 0.76 mg/kg. Each diet was fed to 6 replicates with 20 largemouth bass with the initial body weight of (12.99±0.01) g. The results showed as follows:fish in Y0 group showed the lowest weight gain rate, feeding rate and feed conversion ratio, which were significantly lower than those of fish in other groups (P<0.05). Plasma alkaline phosphatase (AKP) activity in Y0 group was significantly higher than that in other groups (P<0.05). The content of plasma low density lipoprotein cholesterol (HDL-C) in Y0.5 group was significantly higher than that in other groups (P<0.05). The content of plasma urea nitrogen (UN) in Y2.5 group was significantly higher than that in other groups (P<0.05). Plasma immunoglobulin M (IgM) content in Y2.5 and Y5.0 groups was significantly higher than that in Y0 and Y0.5 groups (P<0.05). Compared with Y0 group, plasma malondialdehyde (MDA) content was significantly decreased (P<0.05), and plasma glutathione peroxidase (GSH-Px) activity was significantly increased by Se-yeast supplementation (P<0.05). Liver selenium content in Y5.0 group was significantly higher than that in Y0 and Y0.5 groups (P<0.05), but had no significant difference compared with Y2.5 group (P>0.05). Daily selenium intake had a significant linear correlation with liver selenium content (P<0.05), and the liver selenium content showed a linear increase with the daily selenium intake increasing. Different degrees of liver histological damage were observed in all groups, and fish fed diets with 0.5 mg/kg Se-yeast relieved the symptom. The present study shows that the diet with 0.5 mg/kg Se-yeast (total Se content is 1.29 mg/kg) can enhance the lipid metabolism and antioxidant response of largemouth bass, and it is safe for largemouth bass. Growth performance, plasma biochemical indices, tissue antioxidant indices and hepatic histology are considered synthetically, the tolerance dose of Se-yeast in diet with 0.76 mg/kg background Se for largemouth bass is 0.5 mg/kg (calculated in Se), that is the maximum recommended level of Se, and the safety margin is 1. Animal protein sources such as fish meal and krill meal contain high level of Se element, which need to be cautiously considered when exogenic Se sources are used in high fish meal diets for aquatic animals.

Cite this article

LI Ning, ZHENG Yinhua, WU Xiufeng, WANG Xin, XUE Min, WU Lixin . Tolerance of Selenium-Yeast in Diets of Largemouth Bass (Micropterus salmoides)[J]. Chinese Journal of Animal Nutrition, 2017 , 29(6) : 1949 -1960 . DOI: 10.3969/j.issn.1006-267x.2017.06.016

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