Effects of Pantothenic Acid Supplementation on Lipid Metabolism in Liver of Growing Rex Rabbits

LIU Lei, ZHAO Nan, ZHANG Bin, LI Fuchang

doi:10.3969/j.issn.1006-267x.2017.06.017

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 6: 1961 - 1968

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.06.017

Molecular Nutrition

Effects of Pantothenic Acid Supplementation on Lipid Metabolism in Liver of Growing Rex Rabbits

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College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2016-12-05

Online published: 2017-06-07

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Abstract

This experiment was conducted to investigate the effects of pantothenic acid supplementation on lipid metabolism in liver of growing Rex rabbits. A total of 160 weaned Rex rabbits with the similar body weight of were randomly divided into 4 groups with 40 replicates per group and 1 rabbit per replicate. Rabbits in the control group were fed a basal diet, and the others in the experimental groups were fed the basal diet supplemented with 10, 20 and 40 mg/kg pantothenic acid, respectively. The pre-test period lasted for 7 days, and the experimental period lasted for 53 days. The results showed that dietary supplemented with 40 mg/kg pantothenic acid significantly increased the scapular fat deposition rate, perigastric fat deposition rate and perirenal fat deposition rate of growing Rex rabbits (P<0.05), and dietary supplemented with 10 to 20 mg/kg pantothenic acid significantly decreased the liver fat droplet content of growing Rex rabbits compared with control group (P<0.05). Dietary pantothenic acid supplemental level had no significant effects on plasma total cholesterol and albumin contents of growing Rex rabbits (P>0.05). With the pantothenic acid supplemental level increasing, the plasma triglyceride content of growing Rex rabbits tended to reduce first and then became stable, and it in 20 and 40 mg/kg supplemental groups was significantly lower than that in control group (P<0.05). Compared with control group, dietary supplemented with 20 to 40 mg/kg pantothenic acid significantly increased the plasma very low density lipoprotein content of growing Rex rabbits (P<0.05). Compared with control group, dietary supplemented with 40 mg/kg pantothenic acid significantly increased the expression levels of hormone-sensitive lipase (HSL) gene in liver and fatty acid transport protein (FATP) gene in skeletal muscle (P<0.05), dietary supplemented with 20 to 40 mg/kg pantothenic acid significantly increased the expression level of carnitine palmityl transferase-1 (CPT1) gene in liver (P<0.05), and dietary supplemented with 10 to 40 mg/kg pantothenic acid significantly increased the expression levels of fatty acid synthase (FAS) gene and CPT1 gene in skeletal muscle (P<0.05). In conclusion, pantothenic acid supplementation affects the lipid metabolism in liver of Rex rabbits. Pantothenic acid with the supplemental level of 40 mg/kg can decrease the lipid deposition in liver and the fat deposition rate of body, and increase the uptake and utilization of fatty acids for skeletal muscle.

Key words： Rex rabbits; pantothenic acid; liver; lipid metabolism

Cite this article

LIU Lei, ZHAO Nan, ZHANG Bin, LI Fuchang . Effects of Pantothenic Acid Supplementation on Lipid Metabolism in Liver of Growing Rex Rabbits[J]. Chinese Journal of Animal Nutrition, 2017 , 29(6) : 1961 -1968 . DOI: 10.3969/j.issn.1006-267x.2017.06.017

References

[1] YAN S,YANG X F,LIU H L,et al.Long-chain acyl-CoA synthetase in fatty acid metabolism involved in liver and other diseases:an update[J].World Journal of Gastroenterology,2015,21(12):3492-3498.

[2] KAMANNA V S,GANJI S H,KASHYAP M L.Recent advances in niacin and lipid metabolism[J].Current Opinion in Lipidology,2013,24(3):239-245.

[3] HALL A M,SMITH A J,BERNLOHR D A.Characterization of the acyl-CoA synthetase activity of purified murine fatty acid transport protein 1[J].Journal of Biological Chemistry,2003,278(44):43008-43013.

[4] BRANCA D,SCUTARI G,SILIPRANDI N.Pantethine and pantothenate effect on the CoA content of rat liver[J].International Journal for Vitamin and Nutrition Research,1984,54(2/3):211-216.

[5] MONTEIRO D C F,PATEL V,BARTLETT C P,et al.The structure of the PanD/PanZ protein complex reveals negative feedback regulation of pantothenate biosynthesis by coenzyme A[J].Chemistry & Biology,2015,22(4):492-503.

[6] 孔敏,王宝维,葛文华,等.泛酸干预脂肪甘油三酯脂肪酶和长链脂酰辅酶A合成酶1基因表达对鹅生长和脂类代谢的反向调控[J].动物营养学报,2016,28(5):1433-1441.

[7] WITTWER C T,BECK S,PETERSON M,et al.Mild pantothenate deficiency in rats elevates serum triglyceride and free fatty acid levels[J].The Journal of Nutrition,1990,120(7):719-725.

[8] CIGALA O,PANCALLO M T,DELLA VALLE M,et al.Simvastatin in the treatment of hypercholesterolemia[J].La Clinica Terapeutica,1991,137(5):333-337.

[9] WONG E H F,CLARK R,LEUNG E,et al.The interaction of RS 25259-197,a potent and selective antagonist,with 5-HT₃ receptors,in vitro[J].British Journal of Pharmacology,1995,114(4):851-859.

[10] YOUSSEF J A,SONG W O,BADR M Z.Mitochondrial,but not peroxisomal,β-oxidation of fatty acids is conserved in coenzyme A-deficient rat liver[J].Molecular and Cellular Biochemistry,1997,175(1/2):37-42.

[11] DE BLAS C,MATEOS G G.Feed formulation[M]//DE BLAS C,WISEMAN J.Nutrition of the rabbit.Wallingford:CAB International,1998:222-232.

[12] WEIBEL E R.Stereological techniques for electron microscopic morphometry[M]//HAYAT M A.Principles and techniques of electron microscopy:biological application.New York:Van Nostrand Rheinhold,1973:237-296.

[13] MUKHERJEE S,KATIYAR S S.Nature of O-phthalaldehyde reaction with pigeon liver fatty acid synthetase[J].Indian Journal of Biochemistry & Biophysics,1999,36(2):63-68.

[14] BRITTON C H,MACKEY D W,ESSER V,et al.Fine chromosome mapping of the genes for human liver and muscle carnitine palmitoyltransferase Ⅰ (CPT1A and CPT1B)[J].Genomics,1997,40(1):209-211.

[15] URBAN T,MIKOLÁSOVÁ R,KUCIEL J,et al.A study of associations of the H-FABP genotypes with fat and meat production of pigs[J].Journal of Applied Genetics,2002,43(4):505-509.

[16] FRAYN K N,ARNER P,YKI-JÄRVINEN H.Fatty acid metabolism in adipose tissue,muscle and liver in health and disease[J].Essays in Biochemistry,2006,42:89-103.

[17] 张肖,王宝维,岳斌,等.泛酸对5-16周龄五龙鹅生长性能、屠宰性能、肌肉品质、营养物质利用率及血清生化指标的影响[J].动物营养学报,2015,27(11):3411-3419.

[18] MCNEIL C J,BEATTIE J H,GORDON M J,et al.Nutritional B vitamin deficiency disrupts lipid metabolism causing accumulation of proatherogenic lipoproteins in the aorta adventitia of ApoE null mice[J].Molecular Nutrition & Food Research,2012,56(7):1122-1130.

[19] RAINA V,GUPTA S,YADAV S,et al.Simvastatin induced neurite outgrowth unveils role of cell surface cholesterol and acetyl CoA carboxylase in SH-SY5Y cells[J].PLoS One,2013,8(9):e74547.

[20] LATA G F,ANDERSON E.Effect of prolonged pantothenic acid deprivation upon cholesterol synthesis in the rat[J].Archives of Biochemistry and Biophysics,1954,53(2):518-520.

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