Effects of High Dietary Iron on Iron Transporter Gene Expressions in Duodenal Mucosa and Tissue Microelement Contents of Broilers

ZOU Yaxue, WANG Qiuyue, NIU Yibing, HE Ying, TANG Jiaming, LYU Lin, ZHANG Liyang, LUO Xugang, LI Sufen

doi:10.3969/j.issn.1006-267x.2017.06.019

2017 , Vol. 29 >Issue 6: 1976 - 1987

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.06.019

Molecular Nutrition

Effects of High Dietary Iron on Iron Transporter Gene Expressions in Duodenal Mucosa and Tissue Microelement Contents of Broilers

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1. Hebei Science and Technology Normal College, Qinhuangdao 066000, China;
2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100093, China

Received date: 2016-11-21

Online published: 2017-06-07

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Abstract

This experiment was conducted to investigate the effects of dietary iron (Fe) content on tissue some important microelements such as Fe, manganese (Mn), copper (Cu) and zinc (Zn) contents and Fe transporter gene expressions in duodenal mucosa of broilers, and to explore the effects of Fe on absorption and metabolism of microelements of broilers and its mechanism. A total of 336 one-day-old Rose-308 male chicks were randomly divided into 4 groups with 6 replicates per group and 14 chicks per replicate. Chicks in control group were fed a basal diet without adding exogenous Fe (measured value of Fe content was 78 mg/kg), while those in Fe added groups were fed experimental diets added with 100, 250 and 500 mg/kg Fe (FeSO₄·7H₂O as Fe source) based on the basal diet (measured value of Fe content was 166, 308 and 579 mg/kg, respectively), respectively. The experiment lasted for 21 days. Chicks were slaughtered on 7, 14 and 21 days of age to analyze the contents of Fe, Mn, Cu and Zn in liver, heart, pancreas, duodenal mucosa and tibia ash and the mRNA expression levels of divalent metal transporter 1 (DMT1) and ferroportin 1 (FPN1) in duodenal mucosa. The results showed as follows:1) chicks in the 500 mg/kg Fe added group had significantly lower average daily gain (ADG) during 1 to 7 days of age and 8 to 14 days of age than that in the other three groups (P<0.10), and chicks in the 250 and 500 mg/kg Fe added groups had significantly lower average daily feed intake (ADFI) during 1 to 7 days of age than that in the other two groups (P<0.10). 2) Plasma total iron binding capacity, hemoglobin concentration (except 7 days of age) and hematocrit in whole blood at 7, 14 and 21 days of age were not significantly affected by dietary Fe content (P>0.10). Plasma Fe content and transferrin saturation at 7, 14 and 21 days of age were significantly affected by dietary Fe content (P<0.10), and they were increased as dietary Fe content increasing. 3) The content of Fe in heart at 7 and 14 days of age and in liver, duodenal mucosa, pancreas and tibia ash at 7, 14 and 21 days of age was increased as dietary Fe content increasing, while the content of Mn in duodenal mucosa, pancreas and tibia ash was decreased. Zn content in pancreas at 7 days of age was significantly decreased by dietary added Fe (P<0.10), but the contents of Zn in pancreas at 14 and 21 days of age and Cu in each analyzed tissue at 7, 14 and 21 days of age were not affected by dietary added Fe (P>0.10). 4) The mRNA expression levels of DMT1 and FPN1 in duodenal mucosa at 7, 14 and 21 days of age were significantly affected by dietary Fe content (P<0.10), and they were decreased as dietary Fe content increasing. These results suggest that high Fe diets may decrease the absorption of Mn and Zn through the down-regulations of DMT1 and FPN1 gene expressions in duodenal mucosa, and then result in the lower depositions of Mn and Zn in tissues of broilers.

Key words： iron; iron transporter gene expression; tissue microelement contents; broilers

Cite this article

ZOU Yaxue, WANG Qiuyue, NIU Yibing, HE Ying, TANG Jiaming, LYU Lin, ZHANG Liyang, LUO Xugang, LI Sufen . Effects of High Dietary Iron on Iron Transporter Gene Expressions in Duodenal Mucosa and Tissue Microelement Contents of Broilers[J]. Chinese Journal of Animal Nutrition, 2017 , 29(6) : 1976 -1987 . DOI: 10.3969/j.issn.1006-267x.2017.06.019

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