Molecular Nutrition

Identifying Cell Signaling Pathway that Mediates Epidermal Growth Factor Regulating Sodium Dependent Phosphate Cotransporters Type Ⅱb Expression in Porcine Intestinal Epithelial Cells

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  • College of Animal Science and Technology, Hunan Agricultural University, Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China

Received date: 2016-12-20

  Online published: 2017-06-07

Abstract

The aim of this study was to elucidate the molecular mechanisms of epidermal growth factor (EGF) down-regulated sodium dependent phosphate cotransporters type Ⅱb (NaPi-Ⅱb) expression in porcine intestinal epithelial cells (IPEC-J2). EGF receptor tyrosine kinase inhibitor (tyrphostin AG 1478), protein kinase A (PKA) inhibitor (H89), protein kinase C (PKC) inhibitor (k4393), p38 inhibitor (SB 203580), extracellular signal regulated kinase (ERK) inhibitor (PD98059) and c-Jun n-terminal kinase (JNK) inhibitor (anisomycin) as well as EGF were used to treat IPEC-J2 cells, respectively, and related signal pathways protein and interest protein (NaPi-Ⅱb) expression levels were determined by Western blot. The results showed as follows, compared with control group, NaPi-Ⅱb expression level was significantly decreased after EGF treatment (P<0.05). Compared with none inhibitor treatments, inhibitors of EGF receptor, PKA, PKC, mitogen activated protein kinase (MAPK)/p38, MAPK/ERK1/2 and MAPK/JNK significantly increased NaPi-Ⅱb expression (P<0.05). Especially, MAPK/ERK1/2 inhibitor significantly reduced the phosphorylation level of MAPK/ERK1/2 at Tyr204 site (P<0.05), and MAPK/JNK inhibitor significantly reduced the phosphorylation level of MAPK/JNK1/2/3 at Thr183 and Tyr185 sites (P<0.05). In summary, all EGF receptor, PKA, PKC, p38, ERK and JNK participate in the inhibitory effects of EGF on NaPi-Ⅱb expression.

Cite this article

XING Tingjie, TANG Xiaopeng, CAO Manhu, FANG Rejun . Identifying Cell Signaling Pathway that Mediates Epidermal Growth Factor Regulating Sodium Dependent Phosphate Cotransporters Type Ⅱb Expression in Porcine Intestinal Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2017 , 29(6) : 1988 -1995 . DOI: 10.3969/j.issn.1006-267x.2017.06.020

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