Molecular Nutrition

Effects of Dietary Energy and Protein Levels on mRNA Expression of Small Peptide and Amino Acid Transporters in Small Intestine of Tan Sheep

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  • College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China

Received date: 2016-12-07

  Online published: 2017-06-07

Abstract

This experiment was conducted to study the effects of dietary energy and protein levels on mRNA expression of small peptide and amino acid transporters in small intestine of Tan sheep. A total of 112 healthy Tan sheep with similarly body weight were randomly divided into 4 groups with 4 replicates per group and 7 sheep per replicate. The standard level diet referenced Feeding Standard of Sheep (NY/T 816-2004), sheep in the 4 groups were fed diet with different energy and protein levels diets:0.84×standard level (group Ⅰ), 0.96×standard level (group Ⅱ), 1.08×standard level (group Ⅲ) and 1.20×standard level (group Ⅳ), respectively. The test period were divided into two stages by body weight of sheep:29 to 35 kg and 36 to 40 kg. At the end of each stage, one sheep was slaughtered at each replicate, and small intestinal samples were collected to study the expression of peptide transporter 1 (PepT1), y+ cationic amino acid transporter 1 (CAT1)and excitatory amino-acid transporter 3 (EAAT3) mRNA by real-time PCR. The results showed as follows:1) at the end of 29 to 35 kg stage, the small intestine PepT1 mRNA expression firstly decreased and then increased with diet energy and protein levels increased, and the small intestine PepT1 mRNA expression in group Ⅱ was significantly higher than that in other three groups (P<0.05); the small intestine CAT1 mRNA expression in group Ⅳ was significantly higher than that in other three groups (P<0.05); the small intestine EAAT3 mRNA expression in group Ⅲ was significantly higher than that in other three groups (P<0.05). 2) At the end of 36 to 40 kg stage, the small intestine PepT1 mRNA expression in group Ⅱ was significantly higher than that in other three groups (P<0.05); the small intestine CAT1 mRNA expression in group Ⅱ was significantly higher than that in group Ⅲ (P<0.05); the small intestine EAAT3 mRNA expression had a significantly rise trend with diet energy and protein levels increased, and the small intestine EAAT3 mRNA expression in group Ⅲ and group Ⅳ was significantly higher than that in group Ⅰ and group Ⅱ (P<0.05). In conclusion, dietary energy and protein levels can significantly affect the mRNA expression of PepT1, CAT1 and EAAT3 in small intestine of Tan sheep, which can change the absorption and utilization of the small peptides and amino acids to adapt to Tan sheep's growth and development.

Cite this article

LU Yanjuan, WANG Xiaofang, ZENG Jie, CHEN Yulin, ZHANG Enping . Effects of Dietary Energy and Protein Levels on mRNA Expression of Small Peptide and Amino Acid Transporters in Small Intestine of Tan Sheep[J]. Chinese Journal of Animal Nutrition, 2017 , 29(6) : 2170 -2178 . DOI: 10.3969/j.issn.1006-267x.2017.06.040

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