This experiment was conducted to study the effects of dietary fiber level during pregnancy on reproductive performance and serum reproductive hormone levels of Shenxian sows. Thirty first farrowing pregnant Shenxian sows were randomly divided into three groups and each group had 5 replicates with 2 sows in each replicate. The experiment was carried out from pregnancy to the end of lactation period. The sows in the 3 groups were fed different fiber level diets during pregnancy: the dietary crude fiber (neutral detergent fiber) level of groups 1, 2 and 3 was 10% (31.58%), 12% (31.96%) and 14% (32.65%), respectively. And sows at lactation period fed the same diet. The results showed as follows: 1)the litter size and number born alive of groups 2 and 3 were significantly higher than those of group 1 (P<0.01). The stillbirth rate of group 2 was significantly lower than that of group 1 (P<0.05). The primary litter weight, primary piglet weight, weaning weight of litter and weaning piglet weight of group 2 were significantly higher than those of groups 1 and 3 (P<0.01). The weaning weight of litter (P<0.01), primary litter weight (P<0.05) and weaning piglet weight (P<0.05) of group 3 was significantly higher than that of group 1. 2) The serum levels of estradiol, progesterone and prolactin of pregnant sows for 85 days were not significantly affected by dietary fiber level (P>0.05). The serum levels of estradiol and prolactin of pregnant sows for 114 days of groups 2 and 3 were significantly higher than those of group 1 (P<0.05), while the serum estradiol level was not significantly different among these groups (P>0.05). Comprehensive analysis of various indicators, the results indicate that when dietary crude fiber (neutral detergent fiber) level during pregnancy is 12% (31.96%), the reproductive performance of Shenxian sows is the best.