Effects of Different Ruminally Degradable Starch Levels in Low Starch Diet on in Vitro Ruminal Fermentation

LUO Guobin, WANG Lijun, LIU Yan, ZHANG Guangning, SUN Kaijing, WANG Xinying, ZHANG Yonggen

doi:10.3969/j.issn.1006-267x.2017.07.033

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 7: 2482 - 2491

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.07.033

Molecular Nutrition

Effects of Different Ruminally Degradable Starch Levels in Low Starch Diet on in Vitro Ruminal Fermentation

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School of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2016-12-29

Online published: 2017-07-05

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Abstract

This trial was performed to examine the effects of different ruminally degradable starch levels in the diet with low corn-based starch on in vitro ruminal fermentation. Three Holstein cows fitted with permanent rumen fistulas were used as the donor of rumen fluid. Diets with different ruminally degradable starch levels were used as substrates, and the changes of gas production and ruminal fermentation parameters during 48 h incubation were determined using in vitro gas production method, and the ruminal microflora change during 24 h incubation was also determined. The results showed as follows: 1) with the ruminally degradable starch level increasing, gas production (GP), potential gas production (a+b) and gas production rate constant for "gas production from the slowly soluble fraction (b)" (c) were linearly increased (P<0.05), the gas production from the immediately soluble fraction (a) was linearly decreased (P<0.05), and the dry matter digestibility was linearly increased after 48 h in vitro incubation (P<0.05). 2) With the ruminally degradable starch level increasing, fermentation fluid microprotein (MCP), acetate, propionate, butyrate and total volatile fatty acids (TVFA) concentrations were linearly increased after 48 h in vitro incubation (P<0.05), but the pH and ammoni anitrogen concentration were not significantly changed (P>0.05). 3) With the ruminally degradable starch level increasing, the relative counts of R. albus and R. amylophilus in fermentation fluid after 24 h in vitro incubation were linearly increased (P<0.05), while the relative counts of R. flavefacien, F. succinogenes, B. fibrisolvens, S. bovis and S. amylolytica had no significant changes (P>0.05). It is concluded that incraesing ruminally degradable starch level can improve ruminal fermentation under the condition of low starch diet.

Key words： ruminally degradable starch; gas production; rumen fermentation; microflora

Cite this article

LUO Guobin, WANG Lijun, LIU Yan, ZHANG Guangning, SUN Kaijing, WANG Xinying, ZHANG Yonggen . Effects of Different Ruminally Degradable Starch Levels in Low Starch Diet on in Vitro Ruminal Fermentation[J]. Chinese Journal of Animal Nutrition, 2017 , 29(7) : 2482 -2491 . DOI: 10.3969/j.issn.1006-267x.2017.07.033

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