Molecular Nutrition

Cloning and Sequence Characteristic Analysis of Growth Hormone/Insulin-Like Growth Factors Axis Key Genes from Paa boulengeri

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  • Chongqing Research Centers of Conservation and Development on Rare & Endangered Aquatic Resources, College of Life Science & Forestry, Chongqing University of Art & Science, Chongqing 402168, China

Received date: 2016-12-07

  Online published: 2017-07-05

Abstract

Growth hormone (GH)/insulin-like growth factors (IGFs) axis was composed of GH system and IGFs system, which can promote cell proliferation, regulate growth and development, regulate physiological metabolism, as well as played an important role in the regulation of growth and development. In order to clear the functional structure and evolution characteristics of GH/IGFs axis from Paa boulengeri and to provide the theoretical basis for regulation study of growth and development for Paa boulengeri, the GH, insulin-like growth factor-Ⅰ (IGF-Ⅰ) and insulin-like growth factor-Ⅱ (IGF-Ⅱ) from Paa boulengeri were cloned and the sequence characteristics of them were analyzed. The results showed as follows: 1) multiple sequence alignment with amphibian model animals revealed that GH, IGF-Ⅰ and IGF-Ⅱ displayed respective genetic polymorphisms within conserved functional domains, and the N-terminal domain of IGF-Ⅱ showed a abbreviated evolution trend. 2) The phylogenic clustering analysis found that IGFs from Paa boulengeri and amphibians were classified to one cluster, and they had a near relationship with bony fish, which indicated IGF-Ⅰ and IGF-Ⅱ were comparatively ancient genes. IGFs from Paa boulengeri had a near relationship with aquatic animals such as frogs and fishes, which implied GH had a trend of convergent evolution. 3) To further define the specific functional sites of above genes, the protein structure of them were analyzed by Swiss-model server software, and some potential functional differentiation sites were found including THR52, LEU53, PHE72, PHE73 and SER74 from IGF-Ⅰ, TYR81, LYS82 and LYS83 from IGF-Ⅱ, and PHE208 from GH. The results indicate that the major genes of GH/IGFs axis from Paa boulengeri are relatively conserved, but their exist some potential functional differentiation sites compared with known model species, which can be as molecular targets for the functional study and genetic characteristics analysis of GH/IGFs axis from Paa boulengeri in future.

Cite this article

LUO Jie, JIANG Yusong, YANG Fan, FAN Wenqiao, LI Xiaoying . Cloning and Sequence Characteristic Analysis of Growth Hormone/Insulin-Like Growth Factors Axis Key Genes from Paa boulengeri[J]. Chinese Journal of Animal Nutrition, 2017 , 29(7) : 2492 -2501 . DOI: 10.3969/j.issn.1006-267x.2017.07.034

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