Effect of Sulfated Polysaccharidses from Tremella and Condonpsis pilosula on T Lymphocyte Proliferation and mRNA Expression Level of Interleukin-2 of Broilers

ZHU Guangshuang, HU Yuanliang, CAO Kan, WANG Benzhong

doi:10.3969/j.issn.1006-267x.2017.07.038

2017 , Vol. 29 >Issue 7: 2535 - 2540

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.07.038

Molecular Nutrition

Effect of Sulfated Polysaccharidses from Tremella and Condonpsis pilosula on T Lymphocyte Proliferation and mRNA Expression Level of Interleukin-2 of Broilers

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1. College of Biological Engineering, Wuhu Institute of Technology, Wuhu 241003, China;
2. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2017-03-23

Online published: 2017-07-05

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Abstract

In order to study the mechanism of sulfated polysaccharidses from Tremella (sTPS_70c) and Condonpsis pilosula (sCPPS_50c) on immunological enhancement activity, the effects of sTPS_70c and sCPPS_50c on T lymphocyte proliferation and the mRNA expression level of interleukin-2 (IL-2) were determined by methyl thiazolyl tetrazolium (MTT) assay and real-time fluorescent quantitative PCR assay while taking the unmodified polysaccharidses from Tremella (TPS_tp) as control. The results showed that in single adding into peripheral lymphocyte, sTPS_70c and sCPPS_50c almost at all concentrations and TPStp only at 3.125 μg/mL could significantly stimulate T lymphocyte proliferation (P<0.05). In simultaneous adding into peripheral lymphocyte with plant haemagglutinin P (PHA-P), sCPPS_50c at 0.391 to 1.563 μg/mL significantly stimulated T lymphocyte proliferation (P<0.05). sTPS_70c and sCPPS_50c also could significantly promote mRNA expression level of IL-2 in T lymphocyte at 1.563 μg/mL (P<0.05), and the promoting effects of sTPS_70c were significantly better than TPS_tp (P<0.05), especially at 1.563 μg/mL, the effect of sTPS_70c was the strongest. These results indicate that sulfated modification can enhance the lymphocyte proliferation and mRNA expression of IL-2, and sTPS_70c presents more stronger action, which is related to the degree of substitution at a centain extent.

Key words： sulfated Tremella polysaccharide; sulfated Codonopsis pilosula polysaccharide; lymphocyte proliferation; interleukin-2

Cite this article

ZHU Guangshuang, HU Yuanliang, CAO Kan, WANG Benzhong . Effect of Sulfated Polysaccharidses from Tremella and Condonpsis pilosula on T Lymphocyte Proliferation and mRNA Expression Level of Interleukin-2 of Broilers[J]. Chinese Journal of Animal Nutrition, 2017 , 29(7) : 2535 -2540 . DOI: 10.3969/j.issn.1006-267x.2017.07.038

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