Effects of Compound Antimicrobial Peptides on Ruminal Fermentation and Enzyme Activities of Goats

GAO Shuang, DENG Junliang, CHEN Yun, YANG Yanyi, LIU Qi, CHEN Chong, REN Zhihua, ZUO Zhicai, WANG Ya, CUI Hengmin

doi:10.3969/j.issn.1006-267x.2017.07.040

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 7: 2548 - 2555

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.07.040

Molecular Nutrition

Effects of Compound Antimicrobial Peptides on Ruminal Fermentation and Enzyme Activities of Goats

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Key Laboratory of Environmental Hazard and Animal Diseases, Key Laboratory of Animal Disease and Human Health, College of Veterinary, Medicine of Sichuan Agricultural University, Chengdu 611130, China

Received date: 2016-12-07

Online published: 2017-07-05

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Abstract

This experiment was evaluated to study the effects of compound antimicrobial peptides (C-AMPs) on ruminal fermentation and enzyme activities of goats fed diets with different concentrates. Eighteen 4-month-old male goats were randomly divided into 3 groups and each group had 6 goats. The groups included control group (group Ⅰ), high-concentrate group (group Ⅱ), and high-concentrate antimicrobial peptide group (group Ⅲ), and fed with 300, 600 and 600 g concentrate per head per day, respectively. Moreover, the concentrate of group Ⅲ supplemented with 3.0 g C-AMPs per head per day. The experiment lasted for 60 days. The results showed as follows: relative to the group Ⅰ, the group Ⅱ showed a significant increase in the concentrations of acetate, total volatile fatty acids (T-VFA), methane (CH₄), ammoniacal nitrogen (NH₃-N), urea nitrogen, microprotein (MCP), the ratio of (acetate+butyrate) to propionate, the activities of xylanase and lipase in rumen fluid (P<0.01 or P<0.05), and showed a significant decrease in the activities of carboxymethyl cellulose (CMCase), β-glucosidase and amylase (P<0.01 or P<0.05), but did not show a significant change in the concentrations of propionate and butyrate, and the activities of pectinase and neutral protease (P>0.05). Relative to the group Ⅱ, the group Ⅲ had no significant change in the concentrations of propionate, butyrate, NH₃-N and the activities of CMCase, pectinase, neutral protease and lipase (P>0.05), but the group Ⅲ showed a significant decrease in the concentrations of acetate, T-VFA, CH₄, urea nitrogen, the ratio of (acetate+butyrate) to propionate and the activity of xylanase (P<0.01 or P<0.05), and showed a significant increase in the concentration of MCP and the activities of β-glucosidase and amylase (P<0.01 or P<0.05). The results indicate that dietary C-AMPs can modulate ruminal fermentation model and enhance feed conversion rate, and it can be applied as an ideal feed additive.

Key words： compound antimicrobial peptides; ruminal fermentation; enzyme activities; goats

Cite this article

GAO Shuang, DENG Junliang, CHEN Yun, YANG Yanyi, LIU Qi, CHEN Chong, REN Zhihua, ZUO Zhicai, WANG Ya, CUI Hengmin . Effects of Compound Antimicrobial Peptides on Ruminal Fermentation and Enzyme Activities of Goats[J]. Chinese Journal of Animal Nutrition, 2017 , 29(7) : 2548 -2555 . DOI: 10.3969/j.issn.1006-267x.2017.07.040

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