Effects of Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation Parameters, Plasma Biochemical Indexes and Nutrient Digestibility of Qianbeima Goats

ZHAO Peng, WU Wenxuan, WU Jiahai, ZHU Lunqin, XIN Hailiang, YUAN Xin, YANG Chunhong, YANG Kang, SUN Lun, HAN E

doi:10.3969/j.issn.1006-267x.2017.07.042

2017 , Vol. 29 >Issue 7: 2565 - 2574

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.07.042

Molecular Nutrition

Effects of Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation Parameters, Plasma Biochemical Indexes and Nutrient Digestibility of Qianbeima Goats

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1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Institute of New Rural Development, Guizhou University, Guiyang 550025, China;
3. Institute of Pratacultural Science of Guizhou Province, Guiyang 550006, China;
4. Qiandongnan National Polytechnic, Kaili 556000, China

Received date: 2016-12-01

Online published: 2017-07-05

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Abstract

The present study was conducted to investigate the effects of non-fiber carbohydrate to neutral detergent fiber ratio (NFC/NDF) on rumen fermentation parameters, plasma biochemical indexes and nutrient digestibility of Qianbeima goats. Six healthy adult Qianbeima goats were selected and allocated to 3 groups with 2 replicates per group and 1 goat per replicate using 3×3 Latin square design. Trial group Ⅰ, trial group Ⅱ, and trial group Ⅲ were fed diet with varying NFC/NDF at 2.14:1.00, 1.05:1.00 and 0.40:1.00 (measured values), respectively. The trial lasted for 45 d consisted of 3 periods with 10 d of pre-trial period and 5 d trial period each. The detecting indexes were as follows: dietary nutrient digestibility, rumen fermentation parameters (pH, buffer capacity, ammonia nitrogen and volatile fatty acid concentrations, and cellulose activity) and plasma biochemical indexes [the contents of lipopolysaccharide, albumin and urea nitrogen glucose, and the activities of hydrogen peroxidase, glutamic-oxal(o)acetic transaminase and superoxide dismutase and glutathione peroxidase]. The results showed as follows: 1) there was no significant difference for dry matter intake among 3 groups (P>0.05); trial group Ⅲ showed significantly lower nutrient digestibility compared with trial group Ⅰ and trial group Ⅱ (P<0.05). 2) Trial group Ⅰ resulted in significantly decreased rumen fluid pH over trial group Ⅱ and trial group Ⅲ (P<0.05); trial group Ⅲ significantly increased rumen buffer capacity compared with trial group Ⅰ (P<0.05), but significantly decreased cellobiase activity and acetic acid concentration compared with trial group Ⅰ(P<0.05), and also significantly decreased total volatile fatty acids and butyrate concentrations compared with the other two groups (P<0.05); trial group Ⅰ, trial group Ⅱ and and trial group Ⅲ resulted in gradually increased acetate to propionate ratio and gradually decreased propionate concentration, and the differences among groups were significant (P<0.05); the activities of carboxymethyl cellulose, xylanase and microcrystalline cellulose in rumen fluid were not significantly affected (P>0.05). 3) Plasma lipopolysaccharide content was gradually significantly decreased in trial group Ⅰ, trial group Ⅱ and trial group Ⅲ (P<0.05), while there were no significant differences for other plasma biochemical indexes (P>0.05). It is indicated from the results that over-high NFC/NDF is not beneficial to rumen fermentation parameters and plasma biochemical index of Qianbeima goats, and 1.05: 1.00 is the appropriate level under the conditions of the present study.

Key words： non-fiber carbohydrate to neutral detergent fiber ratio; rumen fermentation parameter; plasma biochemical index; nutrient digestibility; Qianbeima goat

Cite this article

ZHAO Peng, WU Wenxuan, WU Jiahai, ZHU Lunqin, XIN Hailiang, YUAN Xin, YANG Chunhong, YANG Kang, SUN Lun, HAN E . Effects of Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation Parameters, Plasma Biochemical Indexes and Nutrient Digestibility of Qianbeima Goats[J]. Chinese Journal of Animal Nutrition, 2017 , 29(7) : 2565 -2574 . DOI: 10.3969/j.issn.1006-267x.2017.07.042

References

[1] 陈宁.日粮精粗比对断奶初期犊牛瘤胃内环境及粗饲料降解的影响[D].硕士学位论文.重庆:西南大学,2006.

[2] ROTGER A,FERRET A,CALSAMIGLIA S,et al.Changes in ruminal fermentation and protein degradation in growing Holstein heifers from 80 to 250 kg fed high-concentrate diets with different forage-to-concentrate ratios[J].Journal of Animal Science,2005,83(7):1616-1624.

[3] 孙德成,赵智力,魏曼琳,等.不同精粗料比全混合日粮对奶牛瘤胃指标的影响[J].饲料研究,2008(10):47-50.

[4] 王文奇,侯广田,罗永明,等.不同精粗比全混合颗粒饲粮对母羊营养物质表观消化率、氮代谢和能量代谢的影响[J].动物营养学报,2014,26(11):3316-3324.

[5] OWENS F N,SECRIST D S,HILL W J,et al.Acidosis in cattle:a review[J].Journal of Animal Science,1998,76(1):275-286.

[6] 魏德泳,朱伟云,毛胜勇.日粮不同NFC/NDF比对山羊瘤胃发酵与瘤胃微生物区系结构的影响[J].中国农业科学,2012,45(7):1392-1398.

[7] 张立涛,王金文,李艳玲,等.35-50 kg黑头杜泊羊×小尾寒羊F1代杂交羊饲粮中适宜NFC/NDF比例研究[J].中国农业科学,2013,46(21):4620-4632.

[8] 兰云贤.动物饲养标准[M].重庆:西南师范大学出版社,2008.

[9] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.

[10] TUCKER W B,HOGUE J F,ASLAM M,et al.A buffer value index to evaluate effects of buffers on ruminal milieu in cows fed high or low concentrate,silage,or hay diets[J].Journal of Dairy Science,1992,75(3):811-819.

[11] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].内蒙古畜牧科学,1993(4):40-41.

[12] 王加启.反刍动物营养学研究方法[M].北京:现代教育出版社,2011.

[13] 王洪荣,张洁.不同碳水化合物结构日粮对山羊瘤胃发酵和微生物氨基酸组成的影响[J].中国农业科学,2011,44(5):1071-1076.

[14] 徐志军,胡燕,董宽虎.不同精粗比柠条青贮日粮对羔羊生产性能和消化代谢的影响[J].草地学报,2015,23(3):586-593.

[15] 唐志高,雒秋江,闫爱荣,等.妊娠小尾寒羊对3种不同精粗比日粮的消化与代谢[J].新疆农业大学学报,2008,31(1):71-77.

[16] 史仁煌,董双钊,付瑶,等.饲粮中性洗涤纤维水平对泌乳高峰期奶牛生产性能、营养物质表观消化率及血清指标的影响[J].动物营养学报,2015,27(8):2414-2422.

[17] VALDÉS C,CARRO M D,RANILLA M J,et al.Effect of forage to concentrate ratio in complete diets offered to sheep on voluntary food intake and some digestive parameters[J].Animal Science,2000,70(1):119-126.

[18] ANANTASOOK N,WANAPAT M,CHERDTHONG A,et al.Effect of plants containing secondary compounds with palm oil on feed intake,digestibility,microbial protein synthesis and microbial population in dairy cows[J].Asian-Australasian Journal of Animal Sciences,2013,26(6):820-826.

[19] 贾媛媛,王绍庆,常广军,等.高精料诱导的SARA对泌乳期山羊血液和瘤胃液中皮质醇的影响[J].草业学报,2012,21(4):259-266.

[20] 陈安,吴建平,周传社,等.C3和C4植物粗料及底物精粗比对山羊瘤胃体外发酵特性的影响[J].动物营养学报,2015,27(1):112-123.

[21] 刘洁,刁其玉,赵一广,等.饲粮不同NFC/NDF对肉用绵羊瘤胃pH、氨态氮和挥发性脂肪酸的影响[J].动物营养学报,2012,24(6):1069-1077.

[22] MILLER T P,TUCKER W B,LEMA M,et al.Influence of dietary buffer value index on the ruminal milieu of lactating dairy cows fed sorghum silage and grain[J].Journal of Dairy Science,1993,76(11):3571-3579.

[23] 张显东.不同种类粗饲料及添加缓冲剂对绵羊瘤胃发酵的影响[D].硕士学位论文.哈尔滨:东北农业大学,2004.

[24] LABURN H P,FAURIE A,MITCHELL D.The fetus and fever[J].Journal of Thermal Biology,2003,28(2):107-116.

[25] HRISTOV A N,ROPP J K,HUNT C W.Effect of barley and its amylopectin content on ruminal fermentation and bacterial utilization of ammonia-N in vitro[J].Animal Feed Science and Technology,2002,99(1/2/3/4):25-36.

[26] 赵国琦,贾亚红,陈小连,等.不同NDF/NFE比的日粮对山羊瘤胃发酵参数影响的研究[J].中国畜牧杂志,2006,42(13):29-33.

[27] 蔡晶晶,王洪荣,付聪,等.不同NFC/NDF饲粮和硫胺素对奶牛瘤胃代谢的影响[J].动物营养学报,2013,25(9):2012-2020.

[28] AGLE M,HRISTOV A N,ZAMAN S,et al.Effect of dietary concentrate on rumen fermentation,digestibility,and nitrogen losses in dairy cows[J].Journal of Dairy Science,2010,93(9):4211-4222.

[29] RUSSELL J B,WILSON D B.Why are ruminal cellulolytic bacteria unable to digest cellulose at low pH?[J].Journal of Dairy Science,1996,79(8):1503-1509.

[30] 汪水平,王文娟,王加启,等.日粮精粗比对奶牛瘤胃发酵及泌乳性能的影响[J].西北农林科技大学学报:自然科学版,2007,35(6):44-50.

[31] 王海荣.不同日粮精粗比及氮源对绵羊瘤胃纤维降解菌群和纤维物质降解的影响[D].博士学位论文.呼和浩特:内蒙古农业大学,2006.

[32] 张莹莹,王聪,刘强,等.不同精粗比饲粮对晋南牛瘤胃发酵特性和养分消化代谢的影响[J].动物营养学报,2014,26(8):2365-2372.

[33] 朱丹,张佩华,赵勐,等.不同中性洗涤纤维与淀粉比例饲粮对体外瘤胃发酵的影响[J].动物营养学报,2015,27(8):2580-2588.

[34] CHIN A C,FLYNN A N,FEDWICK J P,et al.The role of caspase-3 in lipopolysaccharide-mediated disruption of intestinal epithelial tight junctions[J].Canadian Journal of Physiology and Pharmacology,2006,84(10):1043-1050.

[35] 王林枫,赵志伟,杨改青,等.急性内毒素损伤对奶山羊肝脏营养代谢的影响[J].动物营养学报,2012,24(12):2366-2374.

[36] 胡红莲,刘大程,卢德勋,等.日粮不同非纤维性碳水化合物与中性洗涤纤维的比值对奶山羊瘤胃液和血液中内毒素、组织胺含量的影响[J].中国畜牧兽医,2012,39(3):104-109.

[37] 章森,董国忠,周俊,等.饲粮粗饲料组成和营养水平对奶牛血浆内毒素和代谢产物含量的影响[J].动物营养学报,2014,26(1):227-235.

[38] 崔晓鹏,侯生珍,王志有,等.补饲日粮不同精粗比对妊娠后期藏母羊生产性能及血清指标的影响[J].饲料工业,2016,37(13):40-44.

[39] 门小明.三种精粗比日粮条件下空怀小尾寒羊母羊的消化代谢及部分血液指标的研究[D].硕士学位论文.乌鲁木齐:新疆农业大学,2006.

[40] 燕文平,张莹莹,王聪,等.不同精粗比日粮对肉牛生产性能和血液指标的影响[J].饲料研究,2014(21):54-57.

[41] 徐相亭,王宝亮,程光民,等.不同精粗比日粮对杜泊绵羊生长性能、血清生化指标及经济效益的影响[J].中国畜牧兽医,2016,43(3):668-675.

[42] 施力光,赵春萍,曹婷,等.不同日粮精粗比对海南黑山羊抗氧化性能的影响[J].中国草食动物科学,2015,35(1):29-31.

[43] 吴文旋,段永邦.补饲精料对贵州黑山羊血浆抗氧化应激能力和生化指标的影响[J].家畜生态学报,2013,34(1):60-65.

[44] SGORLON S,STRADAIOLI G,GABAI G,et al.Variation of starch and fat in the diet affects metabolic status and oxidative stress in ewes[J].Small Ruminant Research,2008,74(1/2/3):123-129.

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