This experiment was conducted to investigate the effects of glucose oxidase (GOD) on reproductive performance, antioxidant indexes in serum and dietary nutrient digestibility of Dahe black pregnant sows. Sixty multiparous (3 to 5 parity) healthy Dahe black pregnant sows were randomly divided into four groups with fifteen replicates per group and one sows per replicate. The sows in control group were fed a basal diet, while the others in GOD1, GOD2 and GOD3 groups were fed the basal diet supplemented with 200, 400 and 600 mg/kg GOD, respectively. The experiment period lasted from 30 days before the parturition to weaning of piglets. The results showed as follows: 1) compared with control group, diet supplemented with 400 and 600 mg/kg GOD increased the litter size and number born alive of Dahe black pregnant sows (P>0.05), and diet supplemented with 600 mg/kg GOD significantly increased the birth weight and body weight aged at 20 days of piglets (P<0.05). 2) Compared with control group, diet supplemented with 600 mg/kg GOD significantly increased the activity of glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in serum and significantly decreased the content of malonaldehyde (MDA) in serum of Dahe black pregnant sows and piglets (P<0.05). 3) Compared with control group, diet supplemented with 600 mg/kg GOD significantly increased the dietary ether extract (EE) and phosphorus (P) digestibility of Dahe black pregnant sows (P<0.05). Under this experimental condition, diet supplemented with 600 mg/kg GOD in the later period (30 days before the parturition) of Dahe black pregnant sows can improve reproductive performance of sows, enhance antioxidant capacity of sows and piglets, improve the dietary EE and P digestibility of sows and feed utilization rate.