Molecular Nutrition

Screening of Celluloytic Bacterium from Giant Panda's Feces and Optimization of Condition for Cellulase Production

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  • 1. School of Forestry, Northeast Forestry University, Haerbin 150040, China;
    2. The Second Affiliated Hospital of Harbin Medical University, Haerbin 150086, China;
    3. College of Wildlife Resources, Northeast Forestry University, Haerbin 150040, China

Received date: 2017-02-13

  Online published: 2017-08-02

Abstract

This experiment aimed to screen and identify the cellulose-decomposing bacterium isolated from the feces of giant pandas, and condition for producing cellulase was optimized. The cellulase-producing bacterium named DL was isolated from the feces of giant pandas, used the methods of sodium carboxymethylcellulose (CMC-Na) as unique carbon source and iodine staining, disintegration of filter paper test and measurement of cellulase activity. Based on the morphological observation, physiological and biochemical characteristics and the result of sequences alignment of 16S rDNA, strain DL was identified as Paenibacillus cookii LZ033.The strain of Paenibacillus cookii LZ033 was found to be a spore-forming, gram-positive aerobic bacterium. Medium initial pH, culture temperature, shaker speed and liquid medium volume were chosen to determine the optimum condition for cellulase production. On the basis of the single factor experiment, the orthogonal experiment was used to analysis the optimum condition for cellulase production of strain DL, which was shown as follows: medium initial pH 6, culture temperature 35 ℃, rotation speed 125 r/min, broth liquid medium volume 100 mL in 250 mL flask. Under the optimum condition, the activity of cellulose (expressed in filter paper activity) was up to 102.3 U/mL.

Cite this article

ZHANG Zhi, YIN Wenzhe, YA Nan, MA Jianzhang . Screening of Celluloytic Bacterium from Giant Panda's Feces and Optimization of Condition for Cellulase Production[J]. Chinese Journal of Animal Nutrition, 2017 , 29(8) : 2817 -2825 . DOI: 10.3969/j.issn.1006-267x.2017.08.026

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