Effects of Dietary Level of Methionine on Nutrient Digestion, Gastrointestinal pH and Serum Indexes of Male Hu Lambs

WANG Jie, CUI Kai, WANG Shiqin, DIAO Qiyu, ZHANG Naifeng

doi:10.3969/j.issn.1006-267x.2017.08.046

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 8: 3004 - 3013

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.08.046

Molecular Nutrition

Effects of Dietary Level of Methionine on Nutrient Digestion, Gastrointestinal pH and Serum Indexes of Male Hu Lambs

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Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2017-02-08

Online published: 2017-08-02

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Abstract

The aim of this study was to assess the effects of dietary level of methionine on the nutrient digestion, gastrointestinal pH and serum indexes of male Hu lambs. Twelve pairs of male Hu twin lambs weaned at 7 days old were selected and divided into two groups (control,CON; low methionine level,LM) with a matched-pairs design. During 8 to 56 days, lambs were fed with and basal starter in CON, while LM group ware fed the basal milk replacer and starter deducting 0.70% and 0.4% methionine compared on control group basis, respectively. During 57 to 84 days, all lambs were fed with basal starter. Two periods of were undertaken at 46 to 55 and 74 to 83 days of age using 4 pairs of twin lambs, respectively. Twelve pairs of male Hu twin lambs weaned at 7 days of age were selected and divided into two groups [control (CON) group and low methionine level (LM) group] with a matched-pairs design, and one pair of lambs were assigned to one of the groups. The experiment consisted of two stages. Stage 1 (8 to 56 days of age), lambs in CON group were fed basal milk replacer and basal starter, while those in LM group ware fed basal milk replacer and basal starter deducting 0.70% and 0.40% methionine on the basis of control group, respectively. Stage 2 (57 to 84 days of age), all lambs were stop feeding milk replacer and fed basal starter. Four twins were selected for digestion and metabolism trials at the end of stages 1 (46 to 55 days of age) and 2 (74 to 83 days of age), respectively. The results showed as follows: 1) at 56 days of age, apparent digestibility of crude protein, ether extract and neutral detergent fiber in LM group were significantly lower than those in CON group (P<0.05); at 84 days of age, no significant differences were observed in apparent digestibility of nutrients between groups (P>0.05). 2) At 56 days of age, no significant differences were observed in other gastrointestinal pH between groups (P>0.05) except duodenum pH, which was significantly lower in LM group than CON group (P<0.05); at 84 days of age, no significant differences were observed in gastrointestinal pH between groups (P>0.05). 3) No significant differences were observed in other serum indexes between groups at 56 and 84 days of age (P>0.05) except growth hormone (GH) and insulin (INS) concentrations at 56 days of age, which were significantly lower in LM group than CON group (P<0.05). In conclusion, at 8 to 56 days of age, low dietary methionine level not only decreases apparent digestibility of nutrients, but also decreases duodenum gastrointestinal pH, GH and INS concentrations of male Hu lambs; at 57 to 84 days of age, subsequent recoveries of nutrient apparent digestibility, gastrointestinal pH and serum hormone indexes appear after the compensation of dietary methionine level.

Key words： methionine; lamb; digestion and metabolism; gastrointestinal pH; serum index

Cite this article

WANG Jie, CUI Kai, WANG Shiqin, DIAO Qiyu, ZHANG Naifeng . Effects of Dietary Level of Methionine on Nutrient Digestion, Gastrointestinal pH and Serum Indexes of Male Hu Lambs[J]. Chinese Journal of Animal Nutrition, 2017 , 29(8) : 3004 -3013 . DOI: 10.3969/j.issn.1006-267x.2017.08.046

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