This experiment was conducted to investigate the effects of dietary metabolizable energy level on performance, biochemical indexes in serum and egg quality of Wenshang Luhua hens during laying period, and establish factorial model of metabolizable energy requirement to define dietary metabolizable energy requirements of Wenshang Luhua hens during laying period. Three hundred and sixty 40-week-old healthy Wenshang Luhua hens with similar body weight were randomly allocated to five groups with six replicates per group and twelve hens per replicate according a single-factor completely random design. The hens were fed diets with 10.68, 10.89, 11.10, 11.30, 11.51 MJ/kg metabolizable energy levels, respectively, while the other major nutrient levels were the same. The pretest period lasted for 7 days and the experiment period lasted for 35 days. The results showed as follows:1) dietary metabolizable energy level had significant effects on average daily feed intake (ADFI), average daily metabolizable energy intake (ADMEI), average weight, metabolic weight (BW^0.75), egg number, laying rate and average daily egg mass (ADEM) of Wenshang Luhua hens during laying period (P<0.05). ADFI, ADMEI, egg number, laying rate and ADEM showed an increasing tendency with the increase of dietary metabolizable energy level, but decreased when metabolizable energy level was 11.51 MJ/kg. The ratio of feed to egg showed a decreasing tendency with the increase of dietary metabolizable energy level, but increased when metabolizable energy level was 11.51 MJ/kg. The average weight and BW^0.75 when dietary metabolizable energy level were 11.51 and 11.30 MJ/kg were significantly higher than that when metabolizable energy level was 11.10 MJ/kg (P<0.05). 2) The contents of glucose (GLU), triglyceride (TG) and total cholesterol (TCHO) in serum of Wenshang Luhua hens during laying period showed an increasing tendency with the increase of dietary metabolizable energy level. 3) Dietary metabolizable energy level had significant effects on albumen height, yolk color, Haugh unit and percentage of yolk of Wenshang Luhua hens during laying period (P<0.05). Albumen height and Haugh unit in low energy groups (the metabolizable energy level were 10.68, 10.89 and 11.10 MJ/kg) were significantly higher than that in high energy groups (the metabolizable energy level were 11.30 and 11.51 MJ/kg) (P<0.05). Yolk color and percentage of yolk showed an increasing tendency with the increase of dietary metabolizable energy level, and yolk color and percentage of yolk when metabolizable energy level was 11.51 MJ/kg were significantly higher than that when the other metabolizable energy levels (P<0.05). 4) Given ADMEI as the dependent variable, ADG, ADEM and BW^0.75 as the independent variables, the factorial model of dietary metabolizable energy requirements of Wenshang Luhua hens age from 41 to 45 weeks was ADMEI=0.88ADG+10.63ADEM+476.40BW^0.75 (R²=0.982 5,P<0.05). In conclusion, suitable dietary metabolizable energy requirement for the optimal performance of Wenshang Luhua hens aged from 41 to 45 weeks is 11.29 MJ/kg.