Effects of Methionine Deficiency on Performance, Serum Free Amino Acid Contents and Liver Methionine Metabolism Gene Expression of Laying Hens during Late Period of Laying

LIU Yilin, WU Xin, YIN Yulong, WANG Zhanbin

doi:10.3969/j.issn.1006-267x.2017.09.010

2017 , Vol. 29 >Issue 9: 3091 - 3098

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.09.010

Molecular Nutrition

Effects of Methionine Deficiency on Performance, Serum Free Amino Acid Contents and Liver Methionine Metabolism Gene Expression of Laying Hens during Late Period of Laying

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1. Henan Provincial Academician Workstation of Feed Resource Development and Healthy Livestock, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;
2. Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received date: 2017-02-13

Online published: 2017-09-08

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Abstract

This experiment was conducted to study the effects of methionine deficiency on performance, serum free amino acid contents and liver methionine metabolism gene expression of laying hens during late period of laying. Based on the egg production, a total of 180 Hy-Line Grey laying hens at 62 weeks of age were allocated to 3 groups with 6 replicates each and 10 hens in each replicate. Hens in three groups were fed different diets which contained 0.21%, 0.27% and 0.33% (control group) methionine, respectively, and the experiment period was 90 days. The results showed as follows:1) dietary methionine deficiency significantly reduced the average daily feed intake, laying rate, average daily egg production, average egg weight and unqualified rate of eggs (P<0.05), but significantly increased the ratio of feed to egg of laying hens (P<0.05). 2) Methionine deficiency significantly reduced the serum methionine content of laying hens (P<0.05). The contents of serum serine, glycine and alanine in 0.21% methionine group were significantly higher than those in the other two groups (P<0.05). The contents of serum valine, isoleucine and arginine in 0.27% methionine group were significantly lower than those in control group (P<0.05), and serum proline content in 0.27% methionine group was significantly lower than that in 0.21% methionine group (P<0.05). 3) Compared with control group, the expression levels of DNA methyltransferase 1, N⁶-adenosine-methyltransferase subunit 3 (METTL3) and N⁶-adenosine-methyltransferase subunit 14 in liver of hens in 0.21% methionine group were significantly improved (P<0.05), and the METTL3 expression level in liver of hens in 0.27% methionine group was also significantly improved (P<0.05). 4) Compared with control group, the expression levels of methionine adenosyltransferase 1a, cystathionine-beta-synthase and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) in liver of hens in 0.21% methionine group were significantly improved (P<0.05), and the MTR expression level in liver of hens in 0.27% methionine group was also significantly improved (P<0.05). Methionine deficiency had no significant influence on the expression levels of adenosylhomocysteinas and betaine homocysteine methyltransferase in liver of hens (P>0.05). In conclusion, dietary methionine deficiency reduces performance of hens which may duo to the change of methionine metabolism pathway and the methylation of DNA and RNA.

Key words： methionine; laying hens; performance; methionine metabolism; methylation; gene expression

Cite this article

LIU Yilin, WU Xin, YIN Yulong, WANG Zhanbin . Effects of Methionine Deficiency on Performance, Serum Free Amino Acid Contents and Liver Methionine Metabolism Gene Expression of Laying Hens during Late Period of Laying[J]. Chinese Journal of Animal Nutrition, 2017 , 29(9) : 3091 -3098 . DOI: 10.3969/j.issn.1006-267x.2017.09.010

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