Effects of Jugular-Infused Amino Acid Mixture of Different Profiles on Milk Protein Synthesis of Lactating Dairy Cows

TIAN Wen, DING Luoyang, WU Tianyou, WANG Cuifang, WANG Hongrong, Aochangjin

doi:10.3969/j.issn.1006-267x.2017.09.012

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 9: 3109 - 3119

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.09.012

Molecular Nutrition

Effects of Jugular-Infused Amino Acid Mixture of Different Profiles on Milk Protein Synthesis of Lactating Dairy Cows

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1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Shanghai Bright Holstan Co., Ltd., Shanghai 200436, China;
3. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2017-04-11

Online published: 2017-09-08

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Abstract

This experiment was undertaken to compare the effect of jugular-infused amino acid mixture of casein and ideal profiles on milk yield, milk composition, extraction and utilization of amino acids in mammary gland of mid-lactation Holstein cows. Eight mid-lactation[lactation days:(82±11) d] Holstein cows were used for this study. The dairy cows were randomly assigned to 2 groups by a random block design. The 2 groups were randomly distributed as jugular infusions of 160 g amino acid mixture of casein (Casein group) and ideal profiles (R group), and taken themselves before infusion as blank control group (C1 group was the blank control group for Casein group, and C2 group was the blank control group for R group), respectively. The adaptation period lasted for 14 days and the infusion period lasted for 5 days. Total mixed ration (TMR) was adopted and formulated by corn, soybean meal, cottonseed meal, corn silage, alfalfa hay and Chinese wildrye according to Feeding Standard of Dairy Cow for NRC (2001). The results showed that compared with before infusion, the milk protein yield and content had upward trends after jugular-infused amino acid mixture of casein profile (milk protein yield was increased by 7.14%, P=0.078; milk protein content was increased by 3.27%, P=0.072), in addition, the arterious plasma concentrations of isoleucine (Ile), leucine (Leu), lysine (Lys) and histidine (His) were increased in different extent (Ile concentration was increased by 31.5%, P=0.097; Leu concentration was increased by 65.9%, P=0.041; Lys concentration was increased by 36.9%, P=0.088; His concentration was increased by 40.1%, P=0.010), and the arterious plasma concentrations of threonine (Thr), valine (Val), methionine (Met), phenylalanine (Phe) and arginine (Arg) in Casein group were numerically higher than those in C1 group, but the differences were not significant (P>0.05). The extraction rates of aspartic acid (Asp) and cysteine (Cys) by mammary gland for cows of Casein group were significantly higher than those of C1 group (Asp extraction rate was improved by 95.2%, P=0.031; Cys extraction rate was improved by 49.6%, P=0.031), whereas the extraction rate of glycine (Gly) by mammary gland for cows of Casein group was significantly lower than that in C1 group (decreased by 158.3%, P=0.041). Compared with before infusion, infused amino acid mixture of ideal profile tended to increase the milk protein content (increased by 5.78%, P=0.064) and significantly decrease the milk fat yield (decreased by 8.57%, P=0.015), moreover, there were an upward trend of arterious plasma Arg concentration (increased by 18.0%, P=0.093) and a downward trend of arterious plasma Tyr concentration (decreased by 47.8%, P=0.074) after cows were infused amino acid mixture of ideal profile. After cows were infused of amino acid mixture of ideal profile, the extraction rates of glutamic acid (Glu), Cys and Ile by mammary gland were significantly improved (Glu extraction rate was improved by 118.7%, P=0.015; Cys extraction rate was improved by 77.4%, P=0.032; Ile extraction rate was improved by 46.0%, P=0.012), and the extraction rate of Ser was decreased (decreased by 56.2%, P=0.052). However, the milk fat yield increment (P=0.012) of Casein group was significantly higher than that of R group, and the milk yield increment (P=0.095) and lactose yield increment (P=0.091) tended to be higher for cows receiving amino acid mixture of casein profile than for those receiving amino acid mixture of ideal profile, but the other index increments had no significant differences between Casein and R group (P>0.05). Under this experiment condition, the milk protein content of lactating dairy cows can be increased by the jugular infusions of amino acid mixture of casein and ideal profiles, and the milk protein yield has an upward trend when lactating dairy cows are received jugular infusion of amino acid mixture of casein profile. So, the application effect of jugular-infused amino acid mixture of casein profile is superior to the jugular-infused amino acid mixture of ideal profile.

Key words： casein profile; ideal profile; amino acid mixture; infusion; dairy cows; milk protein

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TIAN Wen, DING Luoyang, WU Tianyou, WANG Cuifang, WANG Hongrong, Aochangjin . Effects of Jugular-Infused Amino Acid Mixture of Different Profiles on Milk Protein Synthesis of Lactating Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2017 , 29(9) : 3109 -3119 . DOI: 10.3969/j.issn.1006-267x.2017.09.012

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