This experiment was conducted to investigate the effects of different contents of corn distillers dried grains with soluble (DDGS) diets supplemented with non-starch polysaccharide enzymes on growth performance, nutrient apparent digestibility coefficients and anti-oxidative ability of juvenile turbot (Scophthalmus maximus L.). Seven hundred and twenty juvenile turbots with the initial body weight of (13.00±0.01) g were randomly assigned to eight groups with three replicates per group and thirty turbots per replicate. D3, D6, D9 and D12 groups were enzyme free control groups, and the fish in them were fed the experimental diets which used corn DDGS to replace 3%, 6%, 9% and 12% fish meal individually. D3+, D6+, D9+ and D12+ groups were adding enzyme experimental groups, and the fish in them were fed the experimental diets which used corn DDGS to replace 3%, 6%, 9% and 12% fish meal individually and added 20 g/t xylanase (120 163 U/g) and 300 g/t cellulase (13 424 U/g). The trial lasted for 9 weeks. The results showed that the final body weight, weight gain rate, specific growth rate and feed efficiency in adding enzyme experimental groups were significantly higher than those in enzyme free control groups when fish fed the same corn DDGS content diet, but the differences were not significant (P>0.05). Feed intake, the contents of moisture, crude protein and crude lipid of whole body, condition factor, hepatosomatic index and viscerosomatic index were not significantly affected by diets supplemented with non-starch polysaccharide enzymes (P>0.05). The apparent digestibility coefficient of dry matter and crude protein in adding enzyme experimental groups were higher than those in enzyme free control groups when fish fed the same corn DDGS content diet. Among them, the apparent digestibility coefficient of dry matter in D9+ group was significantly higher than that in D9 group (P<0.05), the apparent digestibility coefficient of crude protein in D3+ group was significantly higher than that in D3 group (P<0.05), and the apparent digestibility coefficient of crude protein in D12+ group was significantly higher than that in D12 group (P<0.05). When fish fed the same corn DDGS content diet, compared with the enzyme free control groups, the activities of serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in adding enzyme experimental groups were significantly increased (P<0.05), while the content of serum malonaldehyde (MDA) was significantly decreased (P<0.05). In conclusion, the utilization effects of different contents of corn DDGS diets for juvenile turbot are improved by adding non-starch polysaccharide enzymes.