Effects of 6-Benzylaminopurine on Ischemia/Reperfusion Injury of Small Intestine of Rats

LIU Yumei, ZHANG Ziqiang, ZHU Xuemin, WEI Lan, SHI Ke, PAN Li

doi:10.3969/j.issn.1006-267x.2017.09.017

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 9: 3153 - 3158

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.09.017

Molecular Nutrition

Effects of 6-Benzylaminopurine on Ischemia/Reperfusion Injury of Small Intestine of Rats

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College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China

Received date: 2017-03-13

Online published: 2017-09-08

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Abstract

To explore the protective effects of 6-benzylaminopurine (6-BA) on ischemia/reperfusion (I/R) injury of small intestine of rats, eighty male SD rats were selected and randomly divided into four groups named control group, I/R model group, low and high dose 6-BA groups, respectively. Rats in low and high dose 6-BA groups were treated with 10, 20 mg/kg 6-BA by gavage once daily for 3 weeks, and rats in control group and I/R model group were treated with the same volume physiologic saline. Superior mesenteric artery of rats in control group was not blocked after exposed, while it was blocked in I/R model group and low and high dose 6-BA groups for 30 min, and then the blood vessel was reperfused for 60 min. Then the activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), and MDA content in jejunum tissue of rats were measured. Cell DNA damage was observed by single cell gel electrophoresis method, and the expression of cysteinyl aspartate specific proteinase 3 (Caspase-3) was determined by immunity histochemistry method. The results showed as follows:compared with I/R model group, the supplementation of 10 and 20 mg/kg 6-BA significantly increased the activities of T-SOD and GSH-Px (P<0.05), and significantly decreased MDA content (P<0.05); broom shaped tails were less, and DNA content of tails and percentage of broom shaped tails were significantly decreased (P<0.05); Caspase-3 positively expressed cell count was significantly decreased (P<0.05). These results reveal that 6-BA has protective effects against injury of I/R in small intestine, and the effect of 20 mg/kg 6-BA is better.

Key words： 6-benzylaminopurine; ischemia/reperfusion; oxidative damage; apoptosis

Cite this article

LIU Yumei, ZHANG Ziqiang, ZHU Xuemin, WEI Lan, SHI Ke, PAN Li . Effects of 6-Benzylaminopurine on Ischemia/Reperfusion Injury of Small Intestine of Rats[J]. Chinese Journal of Animal Nutrition, 2017 , 29(9) : 3153 -3158 . DOI: 10.3969/j.issn.1006-267x.2017.09.017

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