Molecular Nutrition

Effects of Methionine Tripeptide on Expression Levels of Casein and Small Peptide Transporters Genes in Bovine Mammary Epithelial Cells

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  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2017-03-01

  Online published: 2017-09-08

Abstract

This experiment aimed to study the effects of methionine tripeptide (Met-Met-Met) on expression levels casein and small peptide transporters genes in bovine mammary epithelial cells (BMECs). Using BMECs isolated with collagenase digestion method as the model, different concentrations[0 (control), 40, 50, 60, 70 and 80 μg/mL] of Met-Met-Met were added in culture medium in different treatments and cultured for 24, 48, and 72 h, respectively, and each treatment had 5 replicates with 1 culture pore per replicate. The experiment was repeated twice. Relative growth rate was measured to determine the optimal culture time. Different concentrations[0 (control), 40, 50, 60, 70 and 80 μg/mL] of Met-Met-Met were added in culture medium in different treatments and cultured for the optimal time, and each treatment had 3 replicates with 1 culture pore per replicate. The experiment was repeated for three times. Expression levels of casein genes were determined by real-time PCR to determine the optimal Met-Met-Met concentration. Using the optimal culture time and Met-Met-Met concentration to culture cells, and culture medium without Met-Met-Met was used for control. Each treatment had 3 replicates with 1 culture pore per replicate. The experiment was repeated three times. Expression levels of peptide transporter genes were examined. The results showed as follows:relative growth rate was the highest when cells were treated with Met-Met-Met for 24 h; with the suitable culturing time of 24 h, when adding 60 μg/mL Met-Met-Met, expression levels of αs1-casein and β-casein gens were the highest, meanwhile expression levels of small peptide transporter 1 and 2 genes were significantly higher than those in control treatment (P<0.05). To sum up, adding 60 μg/mL of Met-Met-Met can increase expression levels of casein and peptide transporter genes in BMECs.

Cite this article

GUO Chunli, DAN Ni, CAO Qina, Khas-Erdene, AO Changjin . Effects of Methionine Tripeptide on Expression Levels of Casein and Small Peptide Transporters Genes in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2017 , 29(9) : 3159 -3166 . DOI: 10.3969/j.issn.1006-267x.2017.09.018

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