Molecular Nutrition

Bacteria Diversity in Gastrointestinal Tract of Ailurus fulgens Analyzed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis Technology

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  • 1. Institute of Animal Microecology College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
    2. Chengdu Zoo, Chengdu 610081, China

Received date: 2017-03-29

  Online published: 2017-09-08

Abstract

This study aimed to evaluate the bacterial diversity in the gastrointestinal tract (GIT) of Ailurus fulgens. The polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) technology combined with the cloning sequencing, cluster analysis and principal component analysis (PCA) of bands were used to detect the bacterial composition and diversity. The results showed as follows:1) numerous bacteria were obtained from the GIT of Ailurus fulgens by using the PCR-DGGE profiles. There was a difference in each intestinal segment. However, there were some similarities between the adjacent intestinal segment. Samples from the colon and faeces had the higher bacterial diversity, the secondly for samples from stomach and rectum, and the samples from the jejunum and ileum had the lower bacterial diversity. 2) The sequencing results of PCR-DGGE profiles of bacteria from gastrointestinal tract of Ailurus fulgens showed that most of the bacteria belonged to Firmicutes, Bacteroides, Proteobacteria and Verrucomicrobia. The common bands were mainly inclued uncultured Bacteroidetes bacterium, Enterococcus faecalis, uncultured Clostridium sp., Lactococcus lactis and Weissella cibaria, and Firmicutes was the dominant bacterial. The specific bands were Comamonas sp., Clostridium sp. and Akkermansia. In summary, a large amounts of bacteria exist in the GIT of Ailurus fulgens, and the diversity of bacteria shows a tendency of high-low-high according to the GIT from front to back.

Cite this article

LI Yang, DENG Jiabo, NIU Lili, YU Jianqiu, ZENG Yan, LIU Qian, ZHOU Yi, XIONG Lyuchen, NI Xueqin, ZENG Dong . Bacteria Diversity in Gastrointestinal Tract of Ailurus fulgens Analyzed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis Technology[J]. Chinese Journal of Animal Nutrition, 2017 , 29(9) : 3167 -3174 . DOI: 10.3969/j.issn.1006-267x.2017.09.019

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