Effects of Qi-Zha Oral Solution Residues and Their Fermented Product on Microbes, Microbial Metabolites and Cytokine Expression in Colon of Weaned Piglets

SU Jiayi, GENG Meimei, CHEN Wen, YIN Yulong, WANG Zhanbin, KONG Xiangfeng

doi:10.3969/j.issn.1006-267x.2017.09.027

2017 , Vol. 29 >Issue 9: 3240 - 3247

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.09.027

Molecular Nutrition

Effects of Qi-Zha Oral Solution Residues and Their Fermented Product on Microbes, Microbial Metabolites and Cytokine Expression in Colon of Weaned Piglets

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1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center of Healthy Livestock, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;
3. Hunan Co-Innovation Center of Utilizing Plant Functional Components, Changsha 410128, China

Received date: 2017-02-24

Online published: 2017-09-08

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Abstract

The present study was conducted to determine the effects of Qi-Zha oral solution residues (QOR) and their fermented product on colon micro-ecology and health of weaned piglets, to explore the feasibility of dietary supplementation with herbal residues in piglet production. A total of 120 piglets weaned at 21 days of age were randomly assigned to one of four groups with 5 replicates per group and 6 piglets per replicate, representing blank control group, QOR group (supplementing 5 kg/t QOR), fermented QOR group (supplementing 5 kg/t fermented QOR), and positive control group (supplementing 0.04 kg/t virginiamycin, 0.2 kg/t colistin and 3 000 mg/kg zinc oxide). At day 28 of the experiment, the colonic tissue and its content were collected respectively, for determining the amount of microbes, contents of microbial metabolites and mRNA expression levels of cytokines. The results showed that dietary QOR or fermented QOR did not significantly affect the amounts of seven tested bacteria and total bacteria compared with the blank control group (P>0.05), while significantly decreased the expression level of interleukin (IL)-12 mRNA compared with the two control groups (P<0.05), and significantly decreased indole content compared with the positive control group (P<0.05). Dietary fermented QOR significantly decreased the contents of acetate, butyrate, valerate, isobutyrate, straight chain fatty acids and total short chain fatty acids (P<0.05), as well as the expression level of IL-4 mRNA, when compared with the QOR group (P<0.05). The expression level of granulocyte-macrophage-colony-stimulating factor mRNA was significantly increased (P<0.05), while the amounts of Bacteroidetes and Clostridium cluster ⅩⅣ a were significantly decreased (P<0.05) in the positive control group when compared with the blank control group. Collectively, these findings suggest that the QOR can increase the contents of short chain fatty acids in colonic content and regulate the colon cytokine expression of weaned piglets; antibiotics and zinc oxide, not QOR or fermented QOR, affect the balance of intestinal microflora; the microbe fermentation doesn't enhance the improvement of QOR on intestinal microbe metabolism.

Key words： Qi-Zha oral solution residues; fermented; weaned piglets; colon micro-ecology; cytokine

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SU Jiayi, GENG Meimei, CHEN Wen, YIN Yulong, WANG Zhanbin, KONG Xiangfeng . Effects of Qi-Zha Oral Solution Residues and Their Fermented Product on Microbes, Microbial Metabolites and Cytokine Expression in Colon of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2017 , 29(9) : 3240 -3247 . DOI: 10.3969/j.issn.1006-267x.2017.09.027

References

[1] KWON C H,LEE C Y,HAN S J,et al.Effects of dietary supplementation of lipid-encapsulated zinc oxide on colibacillosis,growth and intestinal morphology in weaned piglets challenged with enterotoxigenic Escherichia coli[J].Animal Science Journal,2014,85(8):805-813.

[2] 黎智华,祝倩,姬玉娇,等.六种中药渣的营养成分[J].天然产物研究与开发,2017,29(1):91-95.

[3] 苏家宜,李华伟,黎智华,等.发酵中药渣对断奶仔猪生长性能和肠黏膜形态结构的影响[J].天然产物研究与开发,2016,28(9):1454-1459.

[4] 刘凤梅,谭显东,羊依金,等.三七渣固态发酵生产蛋白饲料[J].中国酿造,2011(2):67-70.

[5] 李华伟,王宗俊,祝倩,等.饲粮添加发酵中药渣对母猪繁殖性能与子代生长性能的影响[J].天然产物研究与开发,2016,28(10):1534-1539.

[6] 李华伟,黎智华,祝倩,等.饲粮添加中药渣和发酵中药渣对母猪繁殖性能与子代发育的影响[J].动物营养学报,2017,29(1):257-263.

[7] 苏家宜,孔祥峰,李华伟,等.芪楂口服液药渣对断奶仔猪生长性能和肠道健康的影响[J].动物营养学报,2017,29(5):1730-1738.

[8] 敖江涛,郑溜丰,彭健.进程性氧化应激对母猪繁殖性能的影响及其营养调控[J].动物营养学报,2016,28(12):3735-3741.

[9] 焦金真,王芃芃,汤少勋,等.浏阳黑山羊胃肠道不同部位重要功能微生物的数量分布特征研究[J].畜牧兽医学报,2013,44(10):1590-1599.

[10] 耿梅梅,许丽卫,袁红朝,等.气相色谱法测定猪结肠内容物中短链脂肪酸含量[J].现代生物医学进展,2015,15(6):1010-1014.

[11] 姬玉娇,祝倩,耿梅梅,等.高、低营养水平饲粮对环江香猪结肠菌群结构及代谢物的影响[J].微生物学通报,2016,43(7):1650-1659.

[12] 孔祥峰.结肠微生物氮代谢与机体健康研究进展[J].饲料与畜牧,2013(4):10-17.

[13] GUO X,XIA X,TANG R,et al.Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs[J].Letters in Applied Microbiology,2008,47(5):367-373.

[14] 马丽琼,陈德昌,刘绍泽.广谱抗生素对肠道菌群的选择作用[J].中国危重病急救医学,2007,19(8):456-459.

[15] HALES K E,COLE N A,VAREL V H.Effects of corn processing method and dietary inclusion of corn wet distillers grains with solubles on odor and gas production in cattle manure[J].Journal of Animal Science,2012,90(11):3988-4000.

[16] ZHOU Z J,ZHENG W J,SHANG W W,et al.How host gender affects the bacterial community in pig feces and its correlation to skatole production[J].Annals of Microbiology,2015,65(4):2379-2386.

[17] 李彩燕,刘建新,汪海峰.日粮纤维调控猪体粪臭素水平的研究进展[J].中国饲料,2007(9):13-16.

[18] HAN T H,LEE J H,CHO M H,et al.Environmental factors affecting indole production in Escherichia coli[J].Research in Microbiology,2011,162(2):108-116.

[19] KONG X F,JI Y J,LI H W,et al.Colonic luminal microbiota and bacterial metabolite composition in pregnant Huanjiang mini-pigs:effects of food composition at different times of pregnancy[J].Scientific Reports,2016,6:37224.

[20] NICHOLSON J K,HOLMES E,KINROSS J,et al.Host-gut microbiota metabolic interactions[J].Science,2012,336(6086):1262-1267.

[21] JIANG G L,LIU Y Y,OSO A O,et al.The differences of bacteria and bacteria metabolites in the colon between fatty and lean pigs[J].Journal of Animal Science,2016,94(Suppl.3):349-353.

[22] YANG H S,WU F,LONG L N,et al.Effects of yeast products on the intestinal morphology,barrier function,cytokine expression,and antioxidant system of weaned piglets[J].Journal of Zhejiang University:Science B,2016,17(10):752-762.

[23] GAO F,XIE J L,JIA C W,et al.Effects of porcine circovirus type 2 and pseudorabies vaccine co-inoculation on regulatory cytokine mRNA expression in pig peripheral blood mononuclear cells[J].Genetics and Molecular Research,2014,13(1):1540-1547.

[24] HOLMES E,KINROSS J,GIBSON G R,et al.Therapeutic modulation of microbiota-host metabolic interactions[J].Science Translational Medicine,2012,4(137):137rv6.

[25] ISMAIL J,SANKAR J.Systemic inflammatory response syndrome (SIRS) and sepsis-an ever-evolving paradigm[J].The Indian Journal of Pediatrics,2015,82(8):675-676.

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