Effects of Arginine on Immunity of Carp (Cyprinus carpio) in Vivo and in Vitro Experiment

CHENG Zhenyan, QU Mu, SUN Ying, YU Hong, SUN Jinhui, QIAO Xiuting

doi:10.3969/j.issn.1006-267x.2017.09.033

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 9: 3293 - 3300

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.09.033

Molecular Nutrition

Effects of Arginine on Immunity of Carp (Cyprinus carpio) in Vivo and in Vitro Experiment

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1. Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Enterprise Key Lab of the Functional Feed of Aquatic Animals, Tianjin Chenhui Modern Technology Group Co., Ltd., Tianjin 301800, China;
3. Tianjin Ocean Pal Carol Biotech Co., Ltd., Tianjin 300350, China

Received date: 2017-03-02

Online published: 2017-09-08

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Abstract

An in vivo and a in vitro experiment were conducted to evaluate the effects of arginine on immunity of carp. In the in vitro experiment, arginine with the concentrations of 0, 0.5, 1.0, 1.5 and 2.0 mmol/L were added in the culture medium, after different incubation time of kidney leukocytes, the proliferation index, respiratory burst activity, phagocytosis activity and bactericidal rate were assayed respectively. In the in vivo experiment, the carp of average body weight 37 g were divided into 5 groups, and each group had three replicates, each replicate had 10 fish. Arginine at 0, 25, 50, 100, 200 mg/kg were injected into fish cavity, and then fed them with commercial diets for 2 weeks. Then, the proliferation index, respiratory burst activity and phagocytic activity of kidney leukocytes were determined. At the same time, the nitric oxide (NO) content and nitric oxide synthase (NOS) activity in serum and hepatopancreas, and serum albumin (ALB) content were determined. The in vitro experiment results showed that added arginine could increase the proliferation index, respiratory burst activity, phagocytosis activity and bactericidal rate of kidney leukocytes. After 12 and 24 h incubation, the proliferation index of kidney leukocytes in 1.0, 1.5 and 2.0 mmol/L arginine groups was significantly higher than that in 0 mmol/L arginine group (P<0.05); after 6, 12 and 24 h incubation, the respiratory burst activity of kidney leukocytes in 1.0 mmol/L arginine group was significantly higher than that in 0 mmol/L arginine group (P<0.05); after 12 and 24 h incubation, the phagocytosis activity of kidney leukocytes in 1.0, 1.5 and 2.0 mmol/L arginine groups was significantly higher than that in 0 mmol/L arginine group (P<0.05); after 18 h incubation, the bactericidal rate of kidney leukocytes in 1.0, 1.5 and 2.0 mmol/L arginine groups was significantly higher than that in 0 mmol/L arginine group (P<0.05). The in vivo experiment results showed that the proliferation index, respiratory burst activity and phagocytosis activity of kidney leukocytes in 50, 100 and 200 mg/kg arginine groups was significantly higher than that in 0 mg/kg arginine group (P<0.05); the serum ALB and NO contents in 100 and 200 mg/kg arginine groups were significantly higher than those in 0 mg/kg arginine group (P<0.05); the serum NOS activity in 50, 100 and 200 mg/kg arginine groups was significantly higher than that in 0 mg/kg arginine group (P<0.05); the hepatopancreas NO content in 50, 100 and 200 mg/kg arginine groups was significantly higher than that in 0 mg/kg arginine group (P<0.05); the hepatopancreas NOS activity in 25 and 50 mg/kg arginine groups was significantly higher than that in 0 mg/kg arginine group (P<0.05). Generally, the arginine can enhance on the immunity of leukocytes in carp, and the optimum arginine concentration is 1.0 mmol/L in vitro experiment. In normal feeding condition, the optimum arginine injection concentration for carp is 50 to 100 mg/kg in vivo.

Key words： arginine; carp; immunity; cell incubation

Cite this article

CHENG Zhenyan, QU Mu, SUN Ying, YU Hong, SUN Jinhui, QIAO Xiuting . Effects of Arginine on Immunity of Carp (Cyprinus carpio) in Vivo and in Vitro Experiment[J]. Chinese Journal of Animal Nutrition, 2017 , 29(9) : 3293 -3300 . DOI: 10.3969/j.issn.1006-267x.2017.09.033

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