This experiment was conducted to study the effects of diets supplemented with ginseng polysaccharide on performance, serum biochemical indexes and intestinal morphology of male minks during winter hair period. Forty healthy underyearling short hair black male minks at the age of 120 days were randomly divided into 4 groups with 10 replicates per group and 1 mink per replicate. Minks in group Ⅰ (control group) were fed a basal diet, and those in groups Ⅱ, Ⅲ and Ⅳ were fed the basal diet supplemented with 10, 50 and 100 mg/kg ginseng polysaccharide, respectively. The trial lasted for 77 d with 7 d of pretrial period and 70 d of test period. The results showed as follows:diets supplemented with different levels of ginseng polysaccharide had no significant effects on average daily gain, average daily feed intake and feed/gain (P>0.05). Skin length was increased with the ginseng polysaccharide supplemental level increasing, and it in group Ⅳ was significantly higher than that in group Ⅰ (P<0.05); aciculum length in group Ⅳ was the highest and was significantly higher than that in group Ⅰ (P<0.05); villus height in groups Ⅲ and Ⅳ was not significant different (P>0.05), and it in groups Ⅲ and Ⅳ was significantly higher than that in groups Ⅰ and Ⅱ (P<0.05). Diets supplemented with different levels of ginseng polysaccharide had no significant effects on serum protein (total protein, globulin and urea contents) and fat metabolism related indices (total cholesterol and triglyceride contents) (P>0.05), but the total protein had an increase trend with the ginseng polysaccharide supplemental level increasing. Serum alanine aminotransferase (ALT) activity in group Ⅲ was significantly higher than that in groups Ⅰ and Ⅱ (P<0.05), but had no significant difference compared with group Ⅳ (P>0.05); serum aspartate aminotransferase (AST) activity in group Ⅳwas significantly higher than that in groups Ⅰ and Ⅱ (P<0.05), but had no significant difference compared with group Ⅲ (P>0.05); no significant difference was found in serum alkaline phosphatase (ALP) activity among all groups (P>0.05). Serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) contents in groups Ⅱ, Ⅲ and Ⅳ were significantly higher than those in group Ⅰ (P<0.05), but no significant differences were found among groups Ⅱ, Ⅲ and Ⅳ (P>0.05); jejunum villous height and villous height/crypt depth were significantly increased by adding ginseng polysaccharide (P<0.01). In conclusion, diet supplemented with 50 to 100 mg/kg ginseng polysaccharide can improve the intestinal morphology of male minks during winter hair period which benefiting for enhancing the digestion and absorption of nutrients, and enhance the IL-6 and TNF-α contents in serum which benefiting for enhancing the body immunity.