Effects of Lysine on Expressions of Genes and Proteins Involved in Milk Fat Synthesis in Bovine Mammary Epithelial Cells

CHEN Lu, ZHAO Yanli, GUO Xiaoyu, SHI Binlin, YAN Sumei

doi:10.3969/j.issn.1006-267x.2017.09.042

2017 , Vol. 29 >Issue 9: 3366 - 3374

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.09.042

Molecular Nutrition

Effects of Lysine on Expressions of Genes and Proteins Involved in Milk Fat Synthesis in Bovine Mammary Epithelial Cells

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Collage of Animal Science, Inner Mongolia Agriculture University, Hohhot 010018, China

Received date: 2017-02-24

Online published: 2017-09-08

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Abstract

This study was to detect the effects of lysine (Lys) on expressions of genes and proteins involved in milk fat synthesis in bovine mammary epithelial cells (BMECs) and discuss the mechanism of Lys regulating milk fat synthesis. The 3rd passage BMECs were divided into six groups with six replicates per group and one pore per replicate. Cells were cultured in medium containing 0.5 (basal medium, control), 1.0, 2.0, 4.0, 8.0 and 16.0 mmol/L Lys, respectively. The triglyceride (TAG) content, expressions of genes and proteins involved in milk fat synthesis were detected after 48 h cultivation at 37℃ and 5% CO₂. The results showed as follows:TAG content (P=0.013) and gene expressions of fatty acid-binding protein 3 (FABP3, P=0.001), lipoprotein lipase (LPL, P=0.096), fatty acid synthase (FASN, P=0.003), 1-acylglycerol-3-phosphate O-acyltransferase 6 (AGPAT6, P=0.038) and glycerol-3-phosphate acyltrandferase (GPAM, P=0.022) acted dose-dependent on Lys at significant level or significant tendency. Compared with 0.5 mmol/L group, 2.0, 4.0, 8.0 and 16.0 mmol/L groups significantly increased gene expression of FABP3 (P<0.05), and 1.0, 2.0, 4.0, 8.0 and 16.0 mmol/L groups significantly increased gene expression of LPL (P<0.05). Gene expression of FASN in 2.0 mmol/L group was the highest, which was significantly higher than that in 16.0 mmol/L group (P<0.05). Gene expression of stearoyl-CoA desaturase 1 (SCD1) in 2.0 to 4.0 mmol/L groups was significantly higher than that in other groups (P<0.05). Gene expressions of phosphatidic acid phosphatase 1 (LPIN1), butyrophilin subfamily 1 member A1 (BTN1A1) and xanthine dehydrogenase (XDH) in 1.0, 2.0, 4.0 and 8.0 mmol/L groups were significantly higher than those in 0.5 mmol/L group. Compared with the 0.5, 8.0 and 16.0 mmol/L groups, 2.0 and 4.0 mmol/L groups significantly increased gene and protein expression of peroxisome proliferator-activated receptor-γ (PPARγ) (P<0.05). Gene expression of sterol regulatory element binding protein 1 (SREBP1) in 1.0, 2.0 and 4.0 mmol/L groups was significantly higher than that in the other groups (P<0.05), and protein expression of SREBP1 in 1.0 mmol/L group was significantly higher than that in the other groups (P<0.05). High dose of Lys decreased gene expressions of AGPAT6 and GPAM. Compared with 0.5 and 1.0 mmol/L groups, 2.0, 4.0, 8.0 and 16.0 mmol/L groups significantly decreased gene expression of AGPAT6 (P<0.05). Compared with 0.5, 1.0, 2.0 and 4.0 mmol/L groups, 16.0 mmol/L groups significantly decreased gene expression of GPAM (P<0.05). In conclusion, Lys significantly promote milk fat synthesis in BMECs, but high dose of Lys inhibits gene expressions related in milk fat synthesis. Under the conditions in the present study, 2.0 to 4.0 mmol/L of Lys is an optimal level in culture medium.

Key words： dairy cow; bovine mammary epithelial cells; lysine; milk fat

Cite this article

CHEN Lu, ZHAO Yanli, GUO Xiaoyu, SHI Binlin, YAN Sumei . Effects of Lysine on Expressions of Genes and Proteins Involved in Milk Fat Synthesis in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2017 , 29(9) : 3366 -3374 . DOI: 10.3969/j.issn.1006-267x.2017.09.042

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