Molecular Nutrition

Protective Effects of Selenium on Bovine Mammary Epithelial Cells Oxidative Damaged by Lipopolysaccharide

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  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2017-02-27

  Online published: 2017-09-08

Abstract

This experiment was conducted to investigate the protective effects of selenium (Se) on bovine mammary epithelial cells (BMECs) oxidative damaged by lipopolysaccharide (LPS) and the mechanism. The third-generation attachment cultured BMECs were randomly divided into 8 groups with 6 replicates per group and 1 culture pore per replicate. BMECs in control (CON) group were cultured in a basal medium without Se and LPS for 30 h. BMECs in LPS group and Se protection groups were exposed in Se with different concentrations (0, 10, 20, 50, 100, 150 and 200 nmol/L) for 24 h, and then treated with LPS (1 μg/mL) for 6 h. The results showed as follows:1) compared with CON group, the relative proliferation rate in LPS group was significantly decreased (P<0.05), the activities of glutathione peroxidase (GPx), thioredoxin reductase (TrxR), superoxide dismutase (T-SOD) and catalase (CAT), and total antioxidant capacity (T-AOC) in LPS group were significantly decreased (P<0.05), and expressions of genes and proteins of GPx1 and TrxR1 and selenoprotein P (SelP) content in LPS group was significantly decreased (P<0.05); however, nitric oxide (NO) content, activity, gene and protein expressions of inducible nitric oxide synthase (iNOS), contents and gene expressions of inflammatory factors[tumor necrosis factor-alpha (TNF-α), interleukin-1 (IL-1) and interleukin-6 (IL-6)], active oxygen (ROS) activity, and malondialdehyde (MDA) content in LPS group were significantly increased (P<0.05), and gene expressions of factors related to the mitogen-activated protein kinase (MAPK) signaling pathway[p38 mitogen-activated protein kinase (p38MAPK), c-Jun N terminal kinase (JNK) and extracellular signal-regulated kinas 1/2 (ERK1/2)] in LPS group showed the same tendency. 2) Compared with LPS group, with the increase of Se supplemental level in Se protection groups, relative proliferation rate, activities of T-SOD, CAT, GPx and TrxR, T-AOC, gene and protein expressions of GPx1 and TrxR1 were increased first and then decreased; while NO content, activity, gene and protein expressions of iNOS, contents and gene expressions of inflammatory factors (TNF-α, IL-1 and IL-6), gene expressions of factors related to MAPK signal pathway[ERK1/2, JNK and p38MAPK], ROS activity, and MDA content showed the opposite trend; Se at 20 to 100nmol/L showed better protection effects, and in general Se at 50nmol/L showed the best protection effects. The results indicate that Se improves the antioxidant function of BMECs, and protects cells from oxidative damage induced by LPS mainly by increasing TrxR activity, inhibiting the activation of MAPK signaling pathway, and then decreasing NO content, however, high level of Se can cause damage to cells. Se at 20 to 100 nmol/L in culture medium has a better protection effect, and Se at 50 nmol/L has the best effect.

Cite this article

GUO Yongmei, ZHANG Boqi, YAN Sumei, SHI Binlin, GUO Xiaoyu . Protective Effects of Selenium on Bovine Mammary Epithelial Cells Oxidative Damaged by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2017 , 29(9) : 3375 -3384 . DOI: 10.3969/j.issn.1006-267x.2017.09.043

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