Effects of Lentinan on Morphology Structure, Epithelial Cell Number and Tight Junction Protein Expression in Jejunum of Rats against Escherichia coli

JIANG Changsheng, ZHOU Tong, ZHANG Qian, JIN Erhui, CHE Chuanyan, REN Man

doi:10.3969/j.issn.1006-267x.2017.10.020

2017 , Vol. 29 >Issue 10: 3592 - 3597

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.10.020

Molecular Nutrition

Effects of Lentinan on Morphology Structure, Epithelial Cell Number and Tight Junction Protein Expression in Jejunum of Rats against Escherichia coli

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College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China

Received date: 2017-04-17

Online published: 2017-09-28

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Abstract

The study was conducted to investigate the lentinan on morphology structure, epithelial cell number and tight junction protein expression in jejunum of rats against Escherichia coli (E. coli). Twenty-four SD rats were randomly divided into 4 groups (groups A, B, C and D) with 6 replicates per group and 1 rat per replicate. Rats in groups A and B were drank distilled water and rats in groups C and D were drank distilled water supplemented with 20 μg/mL lentinan. In the 15^th days of experiment, rats in groups B and D were administered orally 2 mL E.coli K88 with the concentration of 1×10¹⁰ CFU/mL, meanwhile, rats in groups A and C were given the same amount of normal saline. In the 18th days of experiment, heart blood sampling, collected jejunum fixed and frozen, made paraffin sections and hematoxylin-eosin (HE) staining and periodic acid-schiff (PAS) staining. The villus height, crypt depth, villus width, number of intraepithelial lymphocytes (IEL) and epithelial goblet cells (GC) were measured and the ratio of villus height to crypt depth (V/C) was calculated. The expression of tight junction protein Occludin in jejunum was determined using Western-blot method. The results showed that no significant difference was observed in villus height and villus width among all groups (P>0.05). The crypt depth of group C was significantly lower than that of groups A and B (P<0.01), and significantly lower than that of group C (P<0.05). The V/C of C group was significantly higher than that of groups A, B and D (P<0.01). The IEL number of group C was significantly lower than that of groups B, D (P<0.01), and significantly lower than that of group A (P<0.05). The epithelial GC number of group C was significantly higher than that of groups A and D (P<0.01), and no significant difference compared with that of group B (P>0.05). The Occludin expression of group D was obvious higher than that of groups A, B and C, and the Occludin expression of group B was higher than that of group A. In conclusion, the drinking water of rats supplemented with lentinan can improve the jejunum morphology and enhance the ability of resisting E.coli and promote the expression of Occludin.

Key words： lentinan; Escherichia coli; jejunum; tight junction protein; rats

Cite this article

JIANG Changsheng, ZHOU Tong, ZHANG Qian, JIN Erhui, CHE Chuanyan, REN Man . Effects of Lentinan on Morphology Structure, Epithelial Cell Number and Tight Junction Protein Expression in Jejunum of Rats against Escherichia coli[J]. Chinese Journal of Animal Nutrition, 2017 , 29(10) : 3592 -3597 . DOI: 10.3969/j.issn.1006-267x.2017.10.020

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