Preparation of Microcapsules Prepared with Lactobacillus plantarum and Taishan Pine Pollen Polysaccharide and Its Effects on Growth and Intestinal Environment of Mice

MA Ningning, PEI Zongfei, ZHU Lijun, DONG Wenwen, ZHOU Jianbo, HU Liping, WEI Kai, HUANG He, ZHU Ruiliang

doi:10.3969/j.issn.1006-267x.2017.10.025

2017 , Vol. 29 >Issue 10: 3627 - 3634

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.10.025

Molecular Nutrition

Preparation of Microcapsules Prepared with Lactobacillus plantarum and Taishan Pine Pollen Polysaccharide and Its Effects on Growth and Intestinal Environment of Mice

Expand

1. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
2. Shandong Province Green Food Development Center, Ji'nan 250013, China;
3. Animal Disease Prevention and Control Center of Shandong Province, Ji'nan 250022, China;
4. Shandong New Hope Liuhe Group Co., Ltd., Qingdao 266061, China

Received date: 2017-03-12

Online published: 2017-09-28

Fold

Abstract

Lactobacillus plantarum (L. plantarum), as a kind of microecological feed additive, was easy to be affected by adverse factors such as gastric acid, bile acid and digestive enzyme. Taishan pine pollen polysaccharide (TPPPS) was a kind of water soluble plant polysaccharide, which was easy to be damaged by acid and enzyme. In order to protect the biological activity of L. plantarum and TPPPS and to explore whether they have synergistic effect, this experiment prepared microcapsules with L. plantarum and TPPPS as core materials. The microcapsule embedding process was optimized by orthogonal test, and the performance of the microcapsules prepared under the optimum conditions was determined. Forty-eight specific pathogen free (SPF) mice (weighted about 20 g) were randomly divided into 4 groups with 12 mice per group. Mice in different groups were orally fed 1.0 mL of L. plantarum, L. plantarum+0.5% TPPPS, microcapsules and phosphate buffered saline (PBS) (control group), respectively. The feeding trial lasted for 42 d. Every other week, three mice from each group were selected randomly and weighed. After autopsy, intestinal tissues were aseptically collected and measured the changes of villi and crypts in the small intestine, and then the colonization of lactic acid bacteria and Escherichia coli were determined. The results showed as follows:1) encapsulation efficiency of the microcapsules was 81.6% and diameter was 84.3 μm. Survival rate of L. plantarum was 62.36% after 120 min in simulated gastric juice and 45.9% after 120 days storage at 37℃. 2) compared with control group, L. plantarum, L. plantarum +0.5% TPPPS and the microcapsules could increase body weight of mice at different degrees, increased the number of lactic acid bacteria in small intestine, reduced the number of Escherichia coli, improve development of small intestine villi and crypts, and the effects of the microcapsules were the best. The results indicate that probiotic effect of L. plantarum combined with TPPPS is better than that of L. plantarum alone, while the microcapsules prepared with the above combination can further improve their beneficial effects.

Key words： L. plantarum; Taishan pine pollen polysaccharide; microcapsules; small intestine villi

Cite this article

MA Ningning, PEI Zongfei, ZHU Lijun, DONG Wenwen, ZHOU Jianbo, HU Liping, WEI Kai, HUANG He, ZHU Ruiliang . Preparation of Microcapsules Prepared with Lactobacillus plantarum and Taishan Pine Pollen Polysaccharide and Its Effects on Growth and Intestinal Environment of Mice[J]. Chinese Journal of Animal Nutrition, 2017 , 29(10) : 3627 -3634 . DOI: 10.3969/j.issn.1006-267x.2017.10.025

References

[1] SUO C,YIN Y S,WANG X N,et al.Effects of lactobacillus plantarum ZJ316 on pig growth and pork quality[J].BMC Veterinary Research,2012,8(1):89.

[2] SONG D F,ZHU M Y,GU Q.Purification and characterization of Plantaricin ZJ5,a new bacteriocin produced by Lactobacillus plantarum ZJ5[J].PLoS One,2014,9(8):e105549.

[3] ZHU X,ZHAO Y Z,SUN Y L,et al.Purification and characterisation of plantaricin ZJ008,a novel bacteriocin against Staphylococcus,spp.from Lactobacillus plantarum,ZJ008[J].Food Chemistry,2014,165:216-223.

[4] BECK B R,KIM D,JEON J,et al.The effects of combined dietary probiotics Lactococcus lactis,BFE920 and Lactobacillus plantarum FGL0001 on innate immunity and disease resistance in olive flounder (Paralichthys olivaceus)[J].Fish & Shellfish Immunology,2015,42(1):177-183.

[5] DASH G,RAMAN R P,PANI P K,et al.Evaluation of paraprobiotic applicability of Lactobacillus plantarum in improving the immune response and disease protection in giant freshwater prawn,Macrobrachium rosenbergii (de Man,1879).[J].Fish & Shellfish Immunology,2015,43(1):167-174.

[6] HANDAN A M,EI-SAYED A F,MAHMOUD M M.Effects of a novel marine probiotic,Lactobacillus plantarum AH 78,on growth performance and immune response of Nile tilapia (Oreochromis niloticus)[J].Journal of Applied Microbiology,2016,120(4):1061-1073.

[7] GAEDINER G E,O'SULLIVAN E,KELLY J,et al.Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L.salivarius strains during heat treatment and spray drying[J].Applied and Environmental Microbiology,2000,66(6):2605-2612.

[8] 刘瑛华,赵进宝,吕秀芳.微胶囊包埋技术在益生菌制品中的应用[J].食品与机械,2004,20(2):58-60.

[9] 刘忠杰,徐剑琴.中兽医学[M].北京:中国农业出版社,2003:147-153.

[10] WEI K,SUN Z H,YAN Z G,et al.Effects of Taishan Pinus massoniana pollen polysaccharide on immune response of rabbit haemorrhagic disease tissue inactivated vaccine and on production performance of Rex rabbits[J].Vaccine,2011,29(14):2530-2536.

[11] CUI G L,ZHONG S X,YANG S F,et al.Effects of Taishan Pinus massoniana pollen polysaccharide on the subunit vaccine of Proteus mirabilis in birds[J].International Journal of Biological Macromolecules,2013,56:94-98.

[12] 李宁.双歧杆菌微胶囊制备工艺及功能特性的研究[D].硕士学位论文.保定:河北农业大学,2007.

[13] SHAH N P.Probiotic bacteria:selective enumeration and survival in dairy foods[J].Journal of Dairy Science,2000,83(4):894-907.

[14] PARK J K,CHANG H N.Microencapsulation of microbial cells[J].Biotechnology Advances,2000,18(4):303-319.

[15] EDWARDS A,MUR L A J,GIRDWOOD S E,et al.Coupled cryoconite ecosystem structure-function relationships are revealed by comparing bacterial communities in alpine and Arctic glaciers[J].Fems Microbiology Ecology,2014,89(2):222-237.

[16] FRANCISCO É C,FRANCO T T,WAGNER R,et al.Assessment of different carbohydrates as exogenous carbon source in cultivation of cyanobacteria[J].Bioprocess and Biosystems Engineering,2014,37(8):1497-1505.

[17] 周健,王永坤,张纪兵.复合益生素对肉鸡生产性能和肠道菌群的影响[J].中国家禽,2008,30(19):41-42.

[18] AIDY S E,VAN DEN BOGERT B,KLEEREBEZEM M.The small intestine microbiota,nutritional modulation and relevance for health.[J].Current Opinion in Biotechnology,2015,32:14-20.

[19] CASPARY W F.Physiology and pathophysiology of intestinal absorption[J].American Journal of Clinical Nutrition,1992,55(1):299S-308S.

[20] GARRIGA C,HUNTER R R,AMAT C,et al.Heat stress increases apical glucose transport in the chicken jejunum[J].American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,2006,290(1):R195-R201.

[21] 王永芬,乔宏兴,席磊,等.益生菌-黄芪复合生物制剂的制备及其对肉仔鸡的影响[J].中国农业大学学报,2011,16(1):54-59.

[22] 吕鑫.黄芪多糖和益生菌对海兰褐蛋鸡生产性能和免疫功能的影响[J].饲料博览,2014(12):24-28.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References