Effects of Eucommia ulmoides Leaves on Sheep Lipid Metabolism and the Mechanism

YANG Gaiqing, WANG Linfeng, ZHU Heshui, JIA Shaodan, DU Yinghui, CAO Yuliang, ZHAO Zhiwei, GUO Wenjuan, HU Chang, LI Ming

doi:10.3969/j.issn.1006-267x.2017.10.026

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 10: 3635 - 3647

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.10.026

Molecular Nutrition

Effects of Eucommia ulmoides Leaves on Sheep Lipid Metabolism and the Mechanism

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1. Modern Experimental Techniques and Managing Centre of Henan Agricultural University, Zhengzhou 450002, China;
2. Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China;
3. Luoyang Longxupo Agriculture and Livestock Co., Ltd., Ruyang 471200, China;
4. Ruyang Agriculture and Animal Husbandry Bureau, Ruyang 471200, China;
5. Pingdingshan Animal Husbandry Bureau of Henan Province, Pingdingshan 467000, China;
6. Institute of Jiyuan Animal Health Supervision of Henan Province, Jiyuan 459000, China

Received date: 2017-03-17

Online published: 2017-09-28

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Abstract

In order to research the effects of Eucommia ulmoides leaves (EUL) on sheep lipid metabolism and the mechanism, thirty healthy Hu sheep with the similar age (70 to 80 days of age) and body weight (25 to 30 kg) were randomly divided into three groups with 10 sheep per group, which were control group (CTL group, diets without EUL),low level of EUL group (EUL1 group, diet containing 10% EUL), and high level of EUL group (EUL2 group, diets containing 20% EUL). The pre-experimental period lasted for 15 d, the formal experiment period lasted for 90 d. Blood was collected to determine plasma lipid metabolism indexes; longissimus dorsi muscle was collected to determine nutrient contents; liver tissue was collected to determine mRNA expression levels and protein levels of enzymes or nuclear factors involved in lipid metabolism. The results showed as follows:1) dry matter intake showed EUL1 grouP > EUL2 grouP > CTL group with significant difference among groups (P<0.05). 2) Compared with CTL group, plasma contents of total cholesterol (TC) (P<0.05), low-density lipoprotein (LDL) (0.05 ≤ P<0.10) were reduced, while plasma contents of very low-density lipoprotein (VLDL) (P<0.05), high-density lipoprotein (HDL), non-esterified fatty acid (NEFA) in EUL2 group were significantly increased (P<0.05). 3) Compared with CTL group, crude protein content of muscle in EUL1 group, ether extract content of muscle in EUL2 group were significantly increased (P<0.05), but muscle shear force in EUL1 group was significantly reduced (P<0.05). 4) Compared with CTL group, saturated fatty acids (SFA) content of muscle went down slightly in EUL1 and EUL2 groups (P>0.05), while unsaturated fatty acids (UFA) content was significantly increased in EUL1 and EUL2 groups (P<0.05), in which monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) contents and UFA/SFA in EUL2 group were significantly increased (P<0.05). 5) Compared with CTL group, EUL could significantly down-regulate mRNA expression levels of lipid synthesis related enzyme[stearyl CoA desaturase 1 (SCD1)] and nuclear factors[sterol regulatory element-binding proteins 1c(SREBP-1c) and peroxisome proliferator-activated receptor γ(PPARγ)] in liver (P<0.05), and could significantly up-regulate mRNA expression levels of lipid deposition related enzymes[carnitine palmityl transferase1A (CPT1A) and lipoproteinesterase (LPL)] in liver (P<0.05); compared with CTL group, liver SCD1 protein level in EUL2 group was significantly decreased (P<0.05). These changes indicate that EUL significantly affects lipid metabolism and muscle fatty acid composition of sheep by regulating gene expressions related to lipid synthesis and deposition.

Key words： Eucommia ulmoides leaves; lipid metabolism; plasma biochemical index; fatty acid composition; liver; gene response; sheep

Cite this article

YANG Gaiqing, WANG Linfeng, ZHU Heshui, JIA Shaodan, DU Yinghui, CAO Yuliang, ZHAO Zhiwei, GUO Wenjuan, HU Chang, LI Ming . Effects of Eucommia ulmoides Leaves on Sheep Lipid Metabolism and the Mechanism[J]. Chinese Journal of Animal Nutrition, 2017 , 29(10) : 3635 -3647 . DOI: 10.3969/j.issn.1006-267x.2017.10.026

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