Development of Aflatoxin B1 Colloidal Gold Rapid and Quantitative Test Kit

SONG Qinglong, LI Chenghong, FU Wei, TANG Hongmei, YANG Chunliu, QIAO Shiyan

doi:10.3969/j.issn.1006-267x.2017.10.033

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 10: 3703 - 3709

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.10.033

Molecular Nutrition

Development of Aflatoxin B₁ Colloidal Gold Rapid and Quantitative Test Kit

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1. Beijing Longkefangzhou Bio-Engineering Technoligy Co., Ltd., Beijing 100193, China;
2. National Feed Engineering Technology Research Center, Beijing 100193, China;
3. Chongqing Academy of Animal Sciences, Chongqing 402460, China

Received date: 2017-04-20

Online published: 2017-09-28

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Abstract

This experiment was conducted to develop a colloidal gold rapid and quantitative test kit for detected aflatoxin B₁ content in grain and feed by using colloidal gold immunochromatography technology and colloidal gold quantitative test instrument. The kit using colloidal gold-labeled highly specific monoclonal antibody and conjugated-antigen in an orthogonal experiment to find the optimum condition, meanwhile, by compared the color of the control line and the test line, using the colloidal gold quantitative test instrument to detected the aflatoxin B₁ content in samples. The results showed that the aflatoxin B₁ colloidal gold rapid and quantitative test kit was simple, rapid and stability, the specific data could be obtained, avoided the differences observed by naked eyes. This kit had no cross reaction with common mycotoxin, the relative error of aflatoxin B₁ colloidal gold rapid and quantitative test kit detection results and high performance liquid chromatography detection results was less than 20%. In conclusion, this aflatoxin B₁ colloidal gold rapid and quantitative test kit which developed by the experiment can be used for testing the aflatoxin B1 content in grain and feed rapidly and quantitative.

Key words： aflatoxin B₁; colloidal gold; test paper

Cite this article

SONG Qinglong, LI Chenghong, FU Wei, TANG Hongmei, YANG Chunliu, QIAO Shiyan . Development of Aflatoxin B₁ Colloidal Gold Rapid and Quantitative Test Kit[J]. Chinese Journal of Animal Nutrition, 2017 , 29(10) : 3703 -3709 . DOI: 10.3969/j.issn.1006-267x.2017.10.033

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